Kinetic and stoichiometric characterization of anoxic sulfideoxidation by SO-NR mixed cultures from anoxic biotrickling filters.

Identificadores
URI: http://hdl.handle.net/10498/16298
DOI: 10.1007/s00253-014-5688-5
ISSN: 0175-7598
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2014-01-01Department
Ingeniería Química y Tecnología de AlimentosSource
Applied Microbiology and BiotechnologyAbstract
Monitoring the biological activity in biotrickling
filters is difficult since it implies estimating biomass concentration
and its growth yield, which can hardly be measured in
immobilized biomass systems. In this study, the characterization
of a sulfide-oxidizing nitrate-reducing biomass obtained
from an anoxic biotrickling filter was performed through the
application of respirometric and titrimetric techniques. Previously,
the biomass was maintained in a continuous stirred tank
reactor under steady-state conditions resulting in a growth
yield of 0.328±0.045 g VSS/g S. To properly assess biological
activity in respirometric tests, abiotic assays were conducted
to characterize the stripping of CO2 and sulfide. The
global mass transfer coefficient for both processes was estimated.
Subsequently, different respirometric tests were performed:
(1) to solve the stoichiometry related to the autotrophic
denitrification of sulfide using either nitrate or nitrite as
electron acceptors, (2) to evaluate the inhibition caused by
nitrite and sulfide on sulfide oxidation, and (3) to propose,
calibrate, and validate a kinetic model considering both
electron acceptors in the overall anoxic biodesulfurization
process. The kinetic model considered a Haldane-type equation
to describe sulfide and nitrite inhibitions, a non-competitive
inhibition to reflect the effect of sulfide on the elemental
sulfur oxidation besides single-step denitrification since no
nitrite was produced during the biological assays.
Subjects
Hydrogen sulfide; Biotrickling filter; SO-NR culture; Kinetics and stoichiometry; Respirometry; TitrimetryCollections
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