Optimization of culture media for etanol production from glicerol by Escherichia coli

Identificadores
URI: http://hdl.handle.net/10498/16301
DOI: 10.1016/j.biombioe.2011.12.002
ISSN: 0961-9534
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2012-01-01Department
Ingeniería Química y Tecnología de AlimentosSource
Biomass and Bioenergy - Volume 37, Febrero 2012, pp. 275–281Abstract
The culture media for glycerol to ethanol biotransformation by Escherichia coli under
anaerobic conditions was optimized. A Plackett-Burman screening design was used to
determine which factors were significant with a 95% confidence interval. A full factorial 22
and response surface model were employed to determine the optimum conditions for the
selected response variable. The response variable considered in this work was biomass
productivity (QX). Profiles for biomass growth, glycerol consumption and ethanol production
were obtained at optimum conditions and fermentation parameters were calculated.
Glycerol to ethanol yield and ethanol specific productivity were determined to be 845 g kg 1
of glycerol and 212 g kg 1 h 1 of cell mass respectively. Optimized culture media is presented
along with the main results and experimental profiles obtained from this work.
Subjects
Optimization; Glycerol (propane-1,2,3-triol); Ethanol; Biotransformation; Plackett-Burman; Escherichia coliCollections
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