The Sesquiterpene Synthase from the Botrydial Biosynthetic Gene Cluster of the Phytopathogen Botrytis cinerea

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2008-11-26Department
Química OrgánicaSource
ACS Chemical Biology, 2008, VOL.3 Nº.12, pp. 791–801Abstract
The fungus Botrytis cinerea is the causal agent of the economically
important gray mold disease that affects more than 200 ornamental and agriculturally
important plant species. B. cinerea is a necrotrophic plant pathogen that secretes
nonspecific phytotoxins, including the sesquiterpene botrydial and the
polyketide botcinic acid. The region surrounding the previously characterized
BcBOT1 gene has now been identified as the botrydial biosynthetic gene cluster.
Five genes including BcBOT1 and BcBOT2 were shown by quantitative reverse
transcription-PCR to be co-regulated through the calcineurin signaling pathway. Inactivation
of the BcBOT2 gene, encoding a putative sesquiterpene cyclase, abolished
botrydial biosynthesis, which could be restored by in trans complementation.
Inactivation of BcBOT2 also resulted in overproduction of botcinic acid that
was observed to be strain-dependent. Recombinant BcBOT2 protein converted
farnesyl diphosphate to the parent sesquiterpene of the botrydial biosynthetic
pathway, the tricyclic alcohol presilphiperfolan-8 -ol.
Subjects
botrytis; genCollections
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