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dc.contributor.authorFerreiro-González, Marta
dc.contributor.authorRuiz-Rodríguez, Ana
dc.contributor.authorF. Barbero, Gerardo
dc.contributor.authorCarrera Fernández, Ceferino
dc.contributor.authorAyuso Vilacides, Jesús
dc.contributor.authorPalma Lovillo, Miguel
dc.contributor.authorGarcía Barroso, Carmelo
dc.contributor.otherQuímica Analíticaen_US
dc.contributor.otherQuímica Físicaen_US
dc.date.accessioned2018-04-18T11:07:37Z
dc.date.available2018-04-18T11:07:37Z
dc.date.issued2014-12
dc.identifier.urihttp://hdl.handle.net/10498/20356
dc.description.abstractA method for the concentration and cleaning of red grape extracts prior to the determination of anthocyanins by UPLC-DAD has been developed. This method is of special interest in the determination of phenolic maturity as it allows the analysis of the anthocyanins present in grapes. Several different SPE cartridges were assessed, including both C-18- and vinylbenzene-based cartridges. C-18-based cartridges presented a very low retention for the glucosylated anthocyanidins while vinylbenzene-based cartridges showed excellent retention for these compounds. The optimized method involves the initial conditioning of the cartridge using 10 mL of methanol and 10 mL of water, followed by loading of up to 100 mL of red grape extract. Ten mL of water was used in the washing step and anthocyanins were subsequently eluted using 1.5 mL of acidified methanol at pH 2. This method simplifies the determination of individual anthocyanins as, on the one hand, it cleans the sample of interference and, on the other hand, it increases the concentration to up to 25:1.5. The developed method has been validated with a range of different grapes and it has also been tested as a means of determining the different anthocyanins in grapes with different levels of maturity.en_US
dc.formatapplication/pdfen_US
dc.language.isoengen_US
dc.publisherMoleculesen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourceMolecules 2014, 19, 21398-21410en_US
dc.subjectsolid phase extractionen_US
dc.subjectanthocyaninsen_US
dc.subjectphenolicsen_US
dc.subjectgrapesen_US
dc.titleA New Solid Phase Extraction for the Determination of Anthocyanins in Grapesen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.identifier.doi10.3390/molecules191221398


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This work is under a Creative Commons License Attribution-NonCommercial-NoDerivatives 4.0 Internacional