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dc.contributor.authorS. de Melo, Ivan
dc.contributor.authorJimenez-Nuñez, María D.
dc.contributor.authorIglesias Pérez, Concepción
dc.contributor.authorCampos Caro, Antonio 
dc.contributor.authorMoreno Sánchez, David
dc.contributor.authorRuiz Rodríguez, Félix Alejandro 
dc.contributor.authorBolívar Pérez, Jorge 
dc.contributor.otherBioquímica y Biología Molecular, Microbiología, Medicina Preventiva, Salud Públicaen_US
dc.date.accessioned2018-04-26T09:49:57Z
dc.date.available2018-04-26T09:49:57Z
dc.date.issued2013-03
dc.identifier.urihttp://hdl.handle.net/10498/20409
dc.description.abstractNOA36/ZNF330 is an evolutionarily well-preserved protein present in the nucleolus and mitochondria of mammalian cells. We have previously reported that the pro-apoptotic activity of this protein is mediated by a characteristic cysteine-rich domain. We now demonstrate that the nucleolar localization of NOA36 is due to a highly-conserved nucleolar localization signal (NoLS) present in residues 1–33. This NoLS is a sequence containing three clusters of two or three basic amino acids. We fused the amino terminal of NOA36 to eGFP in order to characterize this putative NoLS. We show that a cluster of three lysine residues at positions 3 to 5 within this sequence is critical for the nucleolar localization. We also demonstrate that the sequence as found in human is capable of directing eGFP to the nucleolus in several mammal, fish and insect cells. Moreover, this NoLS is capable of specifically directing the cytosolic yeast enzyme polyphosphatase to the target of the nucleolus of HeLa cells, wherein its enzymatic activity was detected. This NoLS could therefore serve as a very useful tool as a nucleolar marker and for directing particular proteins to the nucleolus in distant animal species.en_US
dc.formatapplication/pdfen_US
dc.language.isoengen_US
dc.publisherPLOS ONEen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourcePLoS ONE 8(3): e59065en_US
dc.titleNOA36 Protein Contains a Highly Conserved Nucleolar Localization Signal Capable of Directing Functional Proteins to the Nucleolus, in Mammalian Cellsen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.identifier.doi10.1371/journal.pone.0059065
dc.identifier.doi10.1371/journal.pone.0059065


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This work is under a Creative Commons License Attribution-NonCommercial-NoDerivatives 4.0 Internacional