• español
    • English
  • Login
  • English 
    • español
    • English

UniversidaddeCádiz

Área de Biblioteca, Archivo y Publicaciones
Communities and Collections
View Item 
  •   RODIN Home
  • Institutos de Investigación
  • Instituto de Investigación en Biomoléculas INBIO
  • Artículos Científicos INBIO
  • View Item
  •   RODIN Home
  • Institutos de Investigación
  • Instituto de Investigación en Biomoléculas INBIO
  • Artículos Científicos INBIO
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Genetic and Molecular Basis of Botrydial Biosynthesis: Connecting Cytochrome P450-Encoding Genes to Biosynthetic Intermediates

Thumbnail
Identificadores

URI: http://hdl.handle.net/10498/20595

DOI: 10.1021/acschembio.6b00581

Files
2016-Genetic and Molecular Basis of Botrydial Biosynthesis. Connecting Cytochrome P450-Encoding Genes to Biosynthetic Intermediates.pdf (1.011Mb)
Statistics
View statistics
Metrics and citations
 
Share
Export
Export reference to MendeleyRefworksEndNoteBibTexRIS
Metadata
Show full item record
Author/s
Moraga Galindo, JavierAuthority UCA; Dalmais, Bérengère; Izquierdo-Bueno Reina, Inmaculada ConcepciónAuthority UCA; Aleu Casatejada, JosefinaAuthority UCA; R. Hanson, James; Hernández Galán, RosarioAuthority UCA; Viaud, Muriel; González Collado, IsidroAuthority UCA
Date
2016
Department
Química Orgánica
Source
ACS Chemical Biology, 2016, 11, 2838−2846
Abstract
Over two hundred species of plants can be infected by the phytopathogenic fungus Botrytis cinerea under a range of different environmental conditions. In response to these, the fungus produces unique terpenoid and polyketide metabolites. Parts of the plants may be killed by the phytotoxin botrydial, enabling the fungus to feed on the dead cells. In this paper, we describe the genetic and molecular basis of botrydial biosynthesis and the function of the five genes of the genome of B. cinerea that together constitute the botrydial biosynthetic gene cluster. Genes BcBOT3 and BcBOT4, encoding two cytochrome P450 monooxygenases, were inactivated by homologous recombination and were shown to catalyze regio- and stereospecific hydroxylations at the carbons C-10 and C-4, respectively, of the presilphiperfolan-8β-ol skeleton. The null mutants, bcbot3Δ and bcbot4Δ, accumulated key intermediates in the botrydial biosynthesis enabling the complete genetic and molecular basis of the botrydial biosynthetic pathway to be established. Furthermore, the bcbot4Δ mutant overproduced a significant number of polyketides, which included, in addition to known botcinins, botrylactones and cinbotolide A, two new botrylactones and two new cinbotolides, cinbotolides B and C.
Collections
  • Artículos Científicos [4834]
  • Artículos Científicos INBIO [264]
  • Articulos Científicos Quim. Org. [174]
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This work is under a Creative Commons License Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Browse

All of RODINCommunities and CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

Statistics

View Usage Statistics

Información adicional

AboutDeposit in RODINPoliciesGuidelinesRightsLinksStatisticsNewsFrequently Asked Questions

RODIN is available through

OpenAIREOAIsterRecolectaHispanaEuropeanaBaseDARTOATDGoogle Academic

Related links

Sherpa/RomeoDulcineaROAROpenDOARCreative CommonsORCID

RODIN está gestionado por el Área de Biblioteca, Archivo y Publicaciones de la Universidad de Cádiz

Contact informationSuggestionsUser Support