Hoja de datos fenotipos-ratas RYGB -GK and Wistar

Abstract

Roux-en-Y gastric bypass (RYGB) is one of the most effective surgical therapy for the rapid resolution of type 2 diabetes. However, mechanisms underlying entero-hormonal response after surgery and the role of Peptide Tyrosine Tyrosine (PYY) in the restoration of normoglycaemia are still not clear. Using the diabetic Goto-Kakizaki (GK) rat model, we can demonstrate that PYY plasma levels showed a remarkable peak, around thirty minutes earlier than GLP-1 or GIP, after mixed-meal administration in RYGB-operated rats with PYY. GLP-1 and GIP areas under curve (AUC´s) increased after RYGB in GK rats. Also, the findings suggested that PYY (3-36) infusion led to increased GLP-1 and GIP plasma levels close to those obtained after a meal. Finally the number of GLP-1 positive cells number appeared to increase in the three segments of the small intestine in GK RYGB-operated rats beyond early presence of nutrient stimulation in the ileum. But nevertheless, PYY positive cell numbers appeared to increased only in the ileum. Collectively this data demonstrated an earlier central role for PYY in gut hormone regulation after RYGB contributing to GLP-1 and GIP release and support the existence of enteroendocrine communication routes between the distal and proximal small intestine.