Genome and Phylogenetic Analysis of Genes Involved in the Immune System of Solea senegalensis - Potential Applications in Aquaculture

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2019-06Department
Bioquímica y Biología Molecular, Microbiología, Medicina Preventiva, Salud PúblicaSource
Frontiers in Genetics - 2019 Vol. 10 art. 529Abstract
Global aquaculture production continues to increase rapidly. One of the most important
species of marine fish currently cultivated in Southern Europe is Solea senegalensis,
reaching more than 300 Tn in 2017. In the present work, 14 Bacterial Artificial
Chromosome (BAC) clones containing candidate genes involved in the immune system
(b2m, il10, tlr3, tap1, tnfa, tlr8, trim25, lysg, irf5, hmgb2, calr, trim16, and mx), were
examined and compared with other species using multicolor Fluorescence in situ
Hybridization (mFISH), massive sequencing and bioinformatic analysis to determine the
genomic surroundings and syntenic chromosomal conservation of the genomic region
contained in each BAC clone. The mFISH showed that the groups of genes hmgb2-
trim25-irf5-b2m; tlr3-lysg; tnfa-tap1, and il10-mx-trim16 were co-localized on the same
chromosomes. Synteny results suggested that the studied BACs are placed in a smaller
number of chromosomes in S. senegalensis that in other species. Phylogenetic analyses
suggested that the evolutionary rate of immune system genes studied is similar among
the taxa studied, given that the clustering obtained was in accordance with the accepted
phylogenetic relationships among these species. This study contributes to a better
understanding of the structure and function of the immune system of the Senegalese
sole, which is essential for the development of new technologies and products to
improve fish health and productivity.
Subjects
Solea senegalensis; bacterial artificial chromosome; immune system; aquaculture; syntenic conservationCollections
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