A novel magnetic molecularly imprinted polymer for selective extraction and determination of quercetin in plant samples

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URI: http://hdl.handle.net/10498/26492
DOI: 10.1016/j.aca.2022.339709
ISSN: 0003-2670
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2022-04Department
Química AnalíticaSource
Analytica Chimica Acta 1203 (2022) 339709Abstract
Molecularly imprinted polymers that mimic the binding mechanism of antibodies and their antigens
exhibit several advantages, such as fast synthesis, low cost, high stability, and allow to overcome the
ethical issues associated with antibody farming in animals. Herein, a novel strategy combining the
magnetic molecularly imprinted polymer (MMIP) as an artificial antibody with a fluorescence procedure
for the detection of quercetin in plant samples was designed. The MMIP was synthesized via a radical
polymerization process to recognize specific functional groups of quercetin using a green technique
based on high energy ultrasound irradiation. The developed MMIP was characterized by Fourier transform
infrared spectroscopy, X-ray diffraction, scanning/transmission electron microscopy, and thermal
gravimetric analysis, which confirmed the successful preparation of MMIP. The adsorption capacity and
selectivity of the MMIP for quercetin and other interferents analogous were performed. The MMIP was
applied in the solid-phase extraction (SPE) technique as a selective sorbent for the sample preparation.
Besides, a sensitive fluorometric method for the quantitation of quercetin was developed. A linear
response was obtained within the concentration of 0.005e1.25 mg mL 1 of quercetin. The limit of
detection and quantitation were 1.1 ng mL 1 and 3.7 ng mL 1, respectively. The average recoveries for
quercetin were between 92.2% and 104.7% with an RSD less than 5.06% in spiked orange juice and tea
extract samples. Furthermore, the developed procedure was successfully combined with a new paperbased
analytical device for on-site smartphone analysis of quercetin.
© 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND
license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Subjects
Molecularly imprinted polymers; Solid-phase extraction; Magnetic nanoparticles; Quercetin; Fluorescence detection; Paper-based analytical device; High energy ultrasound-based synthesisCollections
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