RT other
T1 Spectrophotometric determination of nitrite in sea water with employment of the standard additions method. Interactive laboratory exercise.
A1 Milla González, Miguel
A2 Química Analítica
K1 quantitative analysis
K1 spectrophotometric methods
K1 spectrophotometry
K1 nitrite
K1 nitrite determination
K1 spectrophotometric determination of nitrite
K1 difenilamine
K1 N (1-naphthyl) ethylenediamine dihydrochloride
K1 standard additions method
K1 SAM
K1 simulated lab exercise
K1 interactive chemistry
K1 analysis of sea water
K1 nitrite in sea water
AB In this exercise, the determination of nitrite in sea water by visible spectrophotometry is carried out. The nitrite anion reacts in acidic medium with sulfanilamide with formation of a diazonium salt, which, in turn, by a coupling reaction with N (1-naphthyl) ethylenediamine dihydrochloride, gives a red azo compound. This azo derivative absorbs at 540 nm. The steps of the analytical procedure are described and the standard additions method (SAM) is employed to find the nitrite concentration in the sample. The data are randomly generated, and the result of the analysis, expressed in ppb of N-NO2-and must be calculated from the equation of the SAM graph, adjusted by least squares. The exercise is a simulation that helps to understand the protocol of analysis and to carry out the procedure in the laboratory session more efficiently.
YR 2014
FD 2014-07-23T00:00:00Z
LK http://hdl.handle.net/10498/16572
UL http://hdl.handle.net/10498/16572
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 23-sep-2023