RT journal article
T1 Use of macroalgal waste from the carrageenan industry as feedstock for the production of polyhydroxybutyrate
A1 Romero Vargas, Agustín
A1 Gallé, Ana
A1 Blandino Garrido, Ana María
A1 Romero García, Luis Isidoro
A2 Ingeniería Química y Tecnología de Alimentos
K1 Cupriavidus necator
K1 enzymatic hydrolysis
K1 industrial macroalgae-based waste
K1 polyhydroxyalkanoates
K1 thermochemical pretreatment
AB The industrial waste generated in the extraction of carrageenan from red seaweed, Eucheuma spinosum, was tested in this study to produce fermentable sugars that could be used for the production of high-value-bioproducts with a biorefinery approach. A sequential process was used: thermochemical pretreatment with HCl and enzymatic hydrolysis. Hydrogen chloride concentrations in the range from 0 mol L−1 to 0.5 mol L−1 and pretreatment times from 15 to 100 min were assayed. The best conditions found for pretreatment were HCl 0.3 mol L−1 for 60 min, leading to reducing sugar concentrations of 21.4 g L−1 (274 mg of reducing sugars per gram of algal residue). The hydrolysates coming from the sequential process under the pretreatment conditions of HCl 0.3 mol L−1 for 60 and 80 min have been used successfully for the production of polyhydroxyalkanoates by Cupriavidus necator. The yields of polyhydroxybutyrate were 0.21–0.26 g PHB g−1 reducing sugar consumed and the accumulation of the biopolymer was of the order of 58% dry cell weight. © 2023 The Authors. Biofuels, Bioproducts and Biorefining published by Society of Industrial Chemistry and John Wiley & Sons Ltd.
PB Wiley
SN 1932-104X
YR 2023
FD 2023-04
LK http://hdl.handle.net/10498/28647
UL http://hdl.handle.net/10498/28647
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 25-may-2026