RT journal article
T1 A lifelong competitive training practice attenuates age-related lipid peroxidation
A1 Barranco-Ruiz, Yaira
A1 Martínez-Amat, Antonio
A1 Casals Vázquez, Cristina
A1 Aragón-Vela, Jerónimo
A1 Rosillo, Silvia
A1 Gomes, Silvana N
A1 Rivas-García, Ana
A1 Guisado, Rafael
A1 Huertas, Jesús R.
A2 Didáctica de la Educación FísicaPlástica y Musical
K1 Aging
K1 Antioxidant defense
K1 Endurance training
K1 Muscle damage
K1 Oxidative stress
AB The effect of exercise-induced oxidative stress on health and aging is not clearly explained. This study examined the effects of habitual sport practice, age, and submaximal exercise on the blood markers of oxidative stress, muscle damage, and antioxidant response. Seventy-two healthy men were grouped by their habitual sport practice: inactive (<1.5 h/week), recreational (3-8 h/week), and trained athletes (>8 h/week), and further divided by age: young (18-25 years), adult (40-55 years), and senior (>55 years). Blood samples were collected at rest and after submaximal effort. Hydroperoxides and superoxide dismutase, glutathione peroxidase, and catalase activities were measured by spectrophotometry. Nuclear DNA damage was analyzed by comet assay. The alpha-actin release was analyzed by Western blot. Alpha-tocopherol, retinol, and coenzyme-Q10 were quantified by high-performance liquid chromatography analysis. Data was analyzed through a factorial ANOVA and the Bonferroni post hoc test. Lipid peroxidation increased significantly with age and submaximal effort (p < 0.05). However, the trained athlete group presented lower lipid peroxidation compared with the recreational group (MD = 2.079, SED = 0.58, p = 0.002) and inactive group (MD = 1.979, SED = 0.61, p = 0.005). Trained athletes showed significant higher alpha-actin levels (p < 0.001) than the other groups. Recreational group showed lower nuclear DNA damage than trained athletes (MD = 3.681, SED = 1.28, p = 0.015). Nevertheless, the inactive group presented significantly higher superoxide dismutase and catalase (p < 0.05) than the other groups. Data suggested that habitual competitive training practice could prevent age-related increases of plasma lipid peroxidation, which, according with our results, cannot be entirely attributed to blood antioxidant defense systems.
PB Springer
SN 1138-7548
YR 2017
FD 2017
LK http://hdl.handle.net/10498/31052
UL http://hdl.handle.net/10498/31052
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 25-may-2026