@misc{10498/22766,
year = {2020},
month = {2},
url = {http://hdl.handle.net/10498/22766},
abstract = {In this work, two methods based on microwave-assisted extraction techniques for the extraction of both anthocyanins and total phenolic compounds from acai have been developed. For that, a full factorial design (Box-Behnken design) has been used to optimize the following four variables: solvent composition (25-75% methanol in water), temperature (50-100 degrees C), pH (2-7), and sample/solvent ratio (0.5 g: 10 mL-0.5 g: 20 mL). The anthocyanins and total phenolic compounds content have been determined by ultra high-pressure liquid chromatography and Folin-Ciocalteu method, respectively. The optimum conditions for the extraction of anthocyanins were 38% MeOH in water, 99.63 degrees C, pH 3.00, at 0.5 g: 10 mL of ratio, while for the extraction of total phenolic compounds they were 74.16% MeOH in water, 99.14 degrees C, pH 5.46, at 0.5 g: 20 mL of ratio. Both methods have shown a high repeatability and intermediate precision with a relative standard deviation lower than 5%. Furthermore, an extraction kinetics study was carried out using extraction periods ranging from 2 min until 25 min. The optimized methods have been applied to acai-containing real samples. The results with such real samples have confirmed that both methods are suitable for a rapid and reliable extraction of anthocyanins and total phenolic compounds.},
publisher = {MDPI},
keywords = {acai},
keywords = {anthocyanins},
keywords = {Box-Behnken design},
keywords = {microwave-assisted extraction},
keywords = {phenolic compounds},
keywords = {UHPLC},
title = {Extraction of Anthocyanins and Total Phenolic Compounds from Açai (Euterpe oleracea Mart.) Using an Experimental Design Methodology. Part 3: Microwave-Assisted Extraction},
doi = {10.3390/agronomy10020179},
author = {Aliaño González, María José and Ferreiro González, Marta and Espada Bellido, Estrella and Carrera Fernández, Ceferino Adrián and Palma Lovillo, Miguel and Ayuso Vilacides, Jesús and Fernández Barbero, Gerardo and Álvarez Saura, José Ángel},
}