@misc{10498/25823,
year = {2021},
month = {11},
url = {http://hdl.handle.net/10498/25823},
abstract = {BACKGROUND: Roux-en-Y gastric bypass (RYGB) is the gold standard method for bariatric surgery and leads to substantial improvements in Type 2 Diabetes mellitus. However, many patients experience relapses in diabetes five years after undergoing this aggressive surgical procedure. We focus on beta-cell population changes and absorptive intestinal consequences after RYGB in a healthy nonobese animal model after a long survival period.

METHODS: For our purpose, we use three groups of Wistar rats: RYGB-operated, surgical control (Sham) and fasting control. We measure alpha-, beta-cell mass; transcription (Arx, and Pdx-1) and proliferation (Ki67) factors; glucose tolerance and insulin release after oral glucose tests; histological adaptive changes in the jejunum; and intestinal glucose transporters.

RESULTS: Our results showed an early increase in insulin secretion after surgery, that decrease at the end of the study. The beta-cell mass reduces twenty-four weeks after RYGB, which coincides with decrease of Pdx-1 transcription promoter factor. These was coincident with an increase in alpha-mass and a high expression of Arx in RYGB group.

CONCLUSIONS: The analysis of all data showed beta-cell mass transdifferentiation into alpha-cell mass in RYGB rats. Due to long-term exhaustion of the beta-cell population by hyperinsulinism derived from digestive tract adaptation to surgery.
Copyright © 2021 The Author(s). Published by Elsevier GmbH.. All rights reserved.},
publisher = {ELSEVIER},
keywords = {Alpha-Cells},
keywords = {Beta-Cell mass},
keywords = {Pancreas},
keywords = {Type 2 Diabetes mellitus},
keywords = {Roux-en Y-gastric bypass},
keywords = {Incretins},
keywords = {Cellular differentiation},
title = {The long-term failure of RYGB surgery in improving T2DM is related to hyperinsulinism.},
doi = {10.1016/j.aanat.2021.151855},
author = {Pérez Arana, Gonzalo Martín and Díaz Gómez, Alfredo Luis and Bancalero-de los Reyes, José and Camacho Ramírez, Alonso and Fernández Vivero, José and Ribelles García, Antonio and Almorza Gomar, David and Carrasco Molinillo, María del Carmen and Mateo-Gavira, Isabel and Prada Oliveira, José Arturo},
}