@misc{10498/25935, year = {2021}, month = {12}, url = {http://hdl.handle.net/10498/25935}, abstract = {Polyhydroxyalkanoates (PHAs) production from lignocellulosic biomass using mixed microbial cultures (MMC) is a potential cheap alternative for reducing the use of petroleum-based plastics. In this study, an MMC adapted to acidogenic effluent from dark fermentation (DF) of exhausted sugar beet cossettes (ESBC) has been tested in order to determine its capability to produce PHAs from nine different synthetic mixtures of volatile fatty acids (VFAs). The tests consisted of mixtures of acetic, propionic, butyric, and valeric acids in the range of 1.5–9.0 g/L of total acidity and with three different valeric:butyric ratios (10:1, 1:1, and 1:10). Experimental results have shown a consistent preference of the MMC for the butyric and valeric acids as carbon source instead other shorter acids (propionic or acetic) in terms of PHA production yield (estimated in dry cell weight basis), with a maximum value of 23% w/w. Additionally, valeric-rich mixtures have demonstrated to carry out a fast degradation process but with poor final PHA production compared with high butyric mixtures. Finally, high initial butyric and valeric concentrations (1.1 g/L and 4.1 g/L) have demonstrated to be counterproductive to PHA production.}, organization = {Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This research was supported by the project CTM2016-79071-R (Spanish Ministry of Economy, Industry and Competitiveness) and financed by the Spanish State Research Agency ("Agencia Estatal de Investigacion" (AEI)) and by the European Regional Development Fund (ERDF) and by grant from the Program for the Promotion and Impulse of Research and Transfer of the University of Cadiz (Ref: IRTP04_UCA).}, publisher = {SPRINGER HEIDELBERG}, keywords = {Polyhydroxyalkanoates}, keywords = {Bioplastic}, keywords = {Volatile fatty acids}, keywords = {Mixed microbial cultures}, title = {Influence of the total concentration and the profile of volatile fatty acids on polyhydroxyalkanoates (PHA) production by mixed microbial cultures}, doi = {10.1007/s13399-021-02208-z}, author = {Bravo Porras, Gloria and Fernández Güelfo, Luis Alberto and Álvarez Gallego, Carlos José and Carbú Espinosa de los Monteros, María and Sales Márquez, Diego and Romero García, Luis Isidoro}, }