@misc{10498/26130,
year = {2021},
month = {11},
url = {http://hdl.handle.net/10498/26130},
abstract = {Nutrient limitation results in an activation of autophagy in organisms ranging from yeast,
nematodes and flies to mammals. Several evolutionary conserved nutrient-sensing kinases are critical
for efficient adaptation of yeast cells to glucose, nitrogen or phosphate depletion, subsequent cellcycle
exit and the regulation of autophagy. Here, we demonstrate that phosphate restriction results in
a prominent extension of yeast lifespan that requires the coordinated activity of autophagy and the
multivesicular body pathway, enabling efficient turnover of cytoplasmic and plasma membrane cargo.
While the multivesicular body pathway was essential during the early days of aging, autophagy
contributed to long-term survival at later days. The cyclin-dependent kinase Pho85 was critical for
phosphate restriction-induced autophagy and full lifespan extension. In contrast, when cell-cycle exit
was triggered by exhaustion of glucose instead of phosphate, Pho85 and its cyclin, Pho80, functioned
as negative regulators of autophagy and lifespan. The storage of phosphate in form of polyphosphate
was completely dispensable to in sustaining viability under phosphate restriction. Collectively, our
results identify the multifunctional, nutrient-sensing kinase Pho85 as critical modulator of longevity
that differentially coordinates the autophagic response to distinct kinds of starvation.},
organization = {This work was supported by the Swedish Research Council (2015-05468 and 2019-05249 to SB), the Knut and AliceWallenberg Foundation (2017.009 to SB), Stiftelsen Olle Engkvist Byggmastare (194-0681 and 207-0527 to SB), the Austrian Science Fund FWF (J4398-B to AA) and Junta de Andalucia (CTS-554 to FAR). IA was a Young Research Staff of the "Sistema de Garantia Juvenil, Fase II" (Junta de Andalucia).},
publisher = {MDPI},
keywords = {lifespan},
keywords = {nutrient limitation},
keywords = {yeast},
keywords = {autophagy},
keywords = {Pho85},
keywords = {aging},
keywords = {polyphosphate},
keywords = {vacuole fusion},
keywords = {quiescence},
title = {Phosphate Restriction Promotes Longevity via Activation of Autophagy and the Multivesicular Body Pathway},
doi = {10.3390/cells10113161},
author = {Ebrahimi, Mahsa and Habernig, Lukas and Broeskamp, Filomena and Aufschnaiter, Andreas and Diessl, Jutta and Atienza Navarro, Isabel and Matz, Steffen and Ruiz Rodríguez, Félix Alejandro and Büttner, Sabrina},
}