@misc{10498/27125,
year = {2022},
month = {5},
url = {http://hdl.handle.net/10498/27125},
abstract = {Simple Summary In catsharks (Scyliorhinus canicula), air exposure induces amino acid catabolism altogether with osmoregulatory imbalances. This study describes a novel NHE isoform being expressed in gills that may be involved in ammonium excretion. Acute-stress situations in vertebrates induce a series of physiological responses to cope with the event. While common secondary stress responses include increased catabolism and osmoregulatory imbalances, specific processes depend on the taxa. In this sense, these processes are still largely unknown in ancient vertebrates such as marine elasmobranchs. Thus, we challenged the lesser spotted catshark (Scyliorhinus canicula) to 18 min of air exposure, and monitored their recovery after 0, 5, and 24 h. This study describes amino acid turnover in the liver, white muscle, gills, and rectal gland, and plasma parameters related to energy metabolism and osmoregulatory imbalances. Catsharks rely on white muscle amino acid catabolism to face the energy demand imposed by the stressor, producing NH4+. While some plasma ions (K+, Cl- and Ca2+) increased in concentration after 18 min of air exposure, returning to basal values after 5 h of recovery, Na+ increased after just 5 h of recovery, coinciding with a decrease in plasma NH4+. These changes were accompanied by increased activity of a branchial amiloride-sensitive ATPase. Therefore, we hypothesize that this enzyme may be a Na+/H+ exchanger (NHE) related to NH4+ excretion. The action of an omeprazole-sensitive ATPase, putatively associated to a H+/K+-ATPase (HKA), is also affected by these allostatic processes. Some complementary experiments were carried out to delve a little deeper into the possible branchial enzymes sensitive to amiloride, including in vivo and ex vivo approaches, and partial sequencing of a nhe1 in the gills. This study describes the possible presence of an HKA enzyme in the rectal gland, as well as a NHE in the gills, highlighting the importance of understanding the relationship between acute stress and osmoregulation in elasmobranchs.},
organization = {This studywas partially supported by grant PID2020-117557RB-C22 (funded byMCIN/AEI/10.13039/501100011033 and by the European Union) and Project DISCARDLIFE (Programa Pleamar, Ministerio de Agricultura y Pesca, Alimentacion y Medio Ambiente, Spain) to JMM. I. Ruiz-Jarabo and J.M. Mancera belong to the Fish Welfare and Stress Network (AGL2016-81808-REDT), supported by the Agencia Estatal de Investigacion (MINECO, Spanish Government).},
publisher = {MDPI},
keywords = {air exposure},
keywords = {amiloride},
keywords = {amino acid consumption},
keywords = {elasmobranchs},
keywords = {NHE},
keywords = {osmoregulation},
keywords = {Scyliorhinus canicula},
keywords = {stress},
title = {Acute Stress in Lesser-Spotted Catshark (Scyliorhinus canicula Linnaeus, 1758) Promotes Amino Acid Catabolism and Osmoregulatory Imbalances},
doi = {10.3390/ANI12091192},
author = {Ruiz Jarabo de la Rocha, Ignacio and Paullada Salmerón, José Antonio and Jerez Cepa, Ismael and Gonçalves Neto, José Belquior and Bystriansky, Jason S. and Mancera Romero, Juan Miguel},
}