@misc{10498/28399,
year = {2023},
month = {2},
url = {http://hdl.handle.net/10498/28399},
abstract = {This study evaluated the feasibility of applying a microalgal peptide-enriched extract, obtained through a sequential alkaline and enzymatic method, as a fundamental media component in Escherichia coli culture. The results demonstrated that the extract is viable as the primary nutrient source for E. coli cultures (wild type), even as a complete substitute for the widely used Luria-Bertani (LB) medium, using an extract dilution of 1:3. Its application in a proportion carbon limiting (dilution 1:7), with glycerol supplementation at the early stationary phase, improved the nutrient use efficiency and controlled the undesirable acetate production. Furthermore, this last strategy applied in an E. coli mutant (M4-Delta iclR) enhanced the production of succinate by 67.3% compared with the M9 medium. The innovative approach proposed in this study opens a wide range of applications and studies related to the production of bio-compounds through diverse microorganisms.},
publisher = {AMER CHEMICAL SOC},
keywords = {microalgae},
keywords = {peptide-enriched extract},
keywords = {culture medium},
keywords = {E. coli},
keywords = {succinate},
title = {Application of a Microalgal Peptide-Enriched Extract as Media Component in E. coli Culture},
doi = {10.1021/acssuschemeng.2c07765},
author = {Callejo-Lopez, J.A. and Ramírez Muñoz, Martín and González Cortés, José Joaquín and Valle Gallardo, Antonio and Cantero Moreno, Domingo and Bolívar Pérez, Jorge},
}