@misc{10498/29445,
year = {2022},
month = {5},
url = {http://hdl.handle.net/10498/29445},
abstract = {The antimicrobial and antileukemic effect of a purified fraction of flavonoids from the leaves of Camellia sinensis was evaluated. An extraction yield of 9.77 mg.g-1 total flavonoids was recovered through a pressurized liquid extraction associated with solid-phase extraction. This fraction was tested against pathogenic microorganisms (Staphylococcus, Salmonella, and Enterococcus), considering the minimum inhibitory concentration. In addition, the human monocyte cell line THP-1, derived from a patient with acute monocytic leukemia, was used for the antitumor assay. The results show that the flavonoid-rich fraction obtained by coupling a Pressurized Liquids Extraction in-line with a Solid Phase Extraction (PLE-SPE) has a high antimicrobial effect and resulted in cell cycle blockage G0 / G1, increased DNA fragmentation, and altered leukemic cell morphology. These results suggest that a flavonoid-rich fraction obtained from Camellia sinensis can be applied as potential adjuvants in chemotherapy treatment to mitigate the side effects caused by chemotherapy or even as a supplement to cancer therapy},
publisher = {Elsevier},
keywords = {Coupled extraction techniques},
keywords = {Flavonoids},
keywords = {Theaflavin},
keywords = {Cancer therapy},
keywords = {THP-1},
title = {Potential application for antimicrobial and antileukemic therapy of a flavonoid-rich fraction of Camellia sinensis},
doi = {10.1016/j.focha.2022.100042},
author = {Corrêa de Souza, Mariana and de Souza Mesquita, Leonardo M. and Pena, Fabíola L. and Tamborlin, Leticia and Capelasso da Silva, Laise and Viganó, Juliane and Costa Antunes, Adriane E and Luchessi, Augusto D. and Teixeira Duarte, Marta C. and Fernández Barbero, Gerardo and Ariel Rostagno, Mauricio},
}