@misc{10498/29780,
year = {2020},
month = {2},
url = {http://hdl.handle.net/10498/29780},
abstract = {Exhausted sugar beet pulp pellets (ESBPP), a sugar industry by-product generated after sugar extraction in the sugar production process, have been used as a raw material for lactic acid (LA) production via hydrolysis and fermentation by Lactobacillus casei. The present study proposes simultaneous saccharification and fermentation (SSF) of ESBPP to design a more cost-effective process. The effects of pH control, nutrient supplementation and solid addition in fed-batch SSF on lactic acid production were investigated.

RESULTS
The highest LA concentration (26.88 g L–1) was reached in fed-batch SSF at a solid/liquid loading of 0.2 g mL–1, with pH control (by adding 30 g L–1 CaCO3 to the medium) and nutrient supplementation (by adding 20 mL of MRS medium per 100 mL of buffer). Under these conditions, a maximum productivity of 0.63 g L−1 h−1 was achieved, 2.7 times higher than that of the control experiment (SSF inoculated at time 0 h). However, a slightly lower LA yield was obtained, revealing the need for an increasing dose of enzymes at high solid-loading SSF.

CONCLUSION
An efficient fed-batch SSF strategy with pH control and MRS supplementation is described in the present study, attaining higher LA productivity compared to separate hydrolysis and fermentation and SSF.},
publisher = {Wiley Online Library},
keywords = {Sugar beet pulp},
keywords = {simultaneous saccharification and fermentation},
keywords = {lactic acid},
keywords = {pH control},
keywords = {fed-batch SSF},
title = {Feasibility of exhausted sugar beet pulp as raw material for lactic acid production},
doi = {https://doi.org/10.1002/jsfa.10334},
author = {Díaz Sánchez, Ana Belén and Blandino Garrido, Ana María and Caro Pina, Ildefonso and Marzo Gago, Cristina and González Pineda, Claudia},
}