@misc{10498/30374,
year = {2018},
month = {1},
url = {http://hdl.handle.net/10498/30374},
abstract = {Hepatitis C virus core protein is a highly basic viral protein that multimerizes with itself to form the viral capsid. When expressed in CD4+ T lymphocytes, it can induce modifications in several essential cellular and biological networks. To shed light on the mechanisms underlying the alterations caused by the viral protein, we have analyzed HCV-core subcellular localization and its associations with host proteins in Jurkat T cells. In order to investigate the intracellular localization of Hepatitis C virus core protein, we have used a lentiviral system to transduce Jurkat T cells and subsequently localize the protein using immunoelectron microscopy techniques. We found that in Jurkat T cells, Hepatitis C virus core protein mostly localizes in the nucleus and specifically in the nucleolus. In addition, we performed pull-down assays combined with Mass Spectrometry Analysis, to identify proteins that associate with Hepatitis C virus core in Jurkat T cells. We found proteins such as NOLC1, PP1γ, ILF3, and C1QBP implicated in localization and/or traffic to the nucleolus. HCV-core associated proteins are implicated in RNA processing and RNA virus infection as well as in functions previously shown to be altered in Hepatitis C virus core expressing CD4+ T cells, such as cell cycle delay, decreased proliferation, and induction of a regulatory phenotype. Thus, in the current work, we show the ultrastructural localization of Hepatitis C virus core and the first profile of HCV core associated proteins in T cells, and we discuss the functions and interconnections of these proteins in molecular networks where relevant biological modifications have been described upon the expression of Hepatitis C virus core protein. Thereby, the current work constitutes a necessary step toward understanding the mechanisms underlying HCV core mediated alterations that had been described in relevant biological processes in CD4+ T cells.},
organization = {Ministry of Education and Science, Spain [SAF2009-09449]; ISCIII [PI16/00784] from the “Plan Estatal de I+D+I 2013-2016/FEDER”; Consejería de Salud de la Junta de Andalucía, Spain [SAS 111206]},
publisher = {FRONTIERS MEDIA SA},
keywords = {Hepatitis C virus},
keywords = {immune evasion},
keywords = {immune tolerance},
keywords = {interactome},
keywords = {proteomics},
keywords = {regulatory T cells},
keywords = {ultrastructure},
title = {Ultrastructural Localization and Molecular Associations of HCV Capsid Protein in Jurkat T Cells.},
doi = {10.3389/fmicb.2017.02595},
author = {Fernández Ponce, Cecilia Matilde and Durán Ruiz, María del Carmen and Narbona Sánchez,  Isaac and Muñoz Miranda, Juan Pedro and Martínez de Arbulo Echevarría, Miguel and Serna-Sanz, Antonio and Baumann, Christian and Litrán Ramos, Rocío and Aguado Vidal, Enrique and Bloch, Wilhelm and García Cózar, Francisco José},
}