@misc{10498/30489,
year = {2017},
month = {2},
url = {http://hdl.handle.net/10498/30489},
abstract = {Endothelial progenitor cells (EPCs) constitute a promising alternative in cardiovascular regenerative medicine
due to their assigned role in angiogenesis and vascular repair. In response to injury, EPCs promote vascular remodeling
by replacement of damaged endothelial cells and/or by secreting angiogenic factors over the damaged
tissue. Nevertheless, such mechanisms need to be further characterized. In the current approach we have evaluated
the initial response of early EPCs (eEPCs) from healthy individuals after direct contact with the factors released
by carotid arteries complicated with atherosclerotic plaques (AP), in order to understand the
mechanisms underlying the neovascularization and remodeling properties assigned to these cells. Herein, we
found that the AP secretome stimulated eEPCs proliferation and mobilization ex vivo, and such increase was accompanied
by augmented permeability, cell contraction and also an increase of cell-cell adhesion in association
with raised vinculin levels. Furthermore, a comparative mass spectrometry analysis of control versus stimulated
eEPCs revealed a differential expression of proteins in the AP treated cells, mostly involved in cell migration, proliferation
and vascular remodeling. Some of these protein changeswere also detected in the eEPCs isolated from
atherosclerotic patients compared to eEPCs from healthy donors.
We have shown, for the first time, that the AP released factors activate eEPCs ex vivo by inducing their mobilization
together with the expression of vasculogenic related markers. The present approach could be taken as a
ex vivo model to study the initial activation of vascular cells in atherosclerosis and also to evaluate strategies
looking to potentiate the mobilization of EPCs prior to clinical applications.},
organization = {Instituto Nacional de Salud Carlos III (PI12-02680 y PI16-00784);  Prime XS-Consortium, EU Seventh Framework Programme
(XS-000158) y European Research Council (ERC Starting Grant).  Andalucía Talent Hub Program_Andalusian Knowledge Agency, co-funded by the EU Seventh Framework Programme (COFUND – Grant n° 291780).},
publisher = {ELSEVIER SCI LTD},
keywords = {Endothelial progenitor cells},
keywords = {Atherosclerosis},
keywords = {Cell therapy},
keywords = {Proteomics},
keywords = {Cell migration},
title = {The atheroma plaque secretome stimulates the mobilization of endothelial progenitor cells ex vivo},
doi = {10.1016/j.yjmcc.2017.02.001},
author = {Vega, Francisco M. and Gautier, Violette and Fernández Ponce, Cecilia Matilde and Extremera, M.J. and Altelaar, A.F.M. and Millan, Jaime and Tellez, Juan C. and Hernandez-Campos, J.A. and Conejero, Rosario and Bolívar Pérez, Jorge and Pardal, R. and García Cózar, Francisco José and Aguado Vidal, Enrique and Heck, Albert J.R. and Durán Ruiz, María del Carmen},
}