@misc{10498/31822,
year = {2023},
url = {http://hdl.handle.net/10498/31822},
abstract = {The present investigation endeavors to optimize a method based on enzyme-assisted extraction for the efficient retrieval of bioactive compounds from mulberry, leveraging its notable health-promoting properties. A combined approach of Plackett–Burman design followed by Box–Behnken design was employed for determining the crucial extraction parameters and subsequently, refining the process. Optimal conditions consisted of heating 0.15 g of mulberry at 40 °C, using 15 mL of 70% EtOH as a solvent at pH 4, 38.46 enzyme units per g of sample, and shaking at 200 rpm. The optimum extraction time study revealed that 5 min of extraction was sufficient to reach the maximum concentration of the bioactive compound. The repeatability and intermediate precision assessment exhibited a coefficient of variation below 5%. Among the diverse mulberry varieties scrutinized, Morus nigra showed the highest anthocyanin content (27.90 ± 2.14 mg/100 g), while Morus rubra showed the highest concentration of phenolic compounds (121.10 ± 19.56 mg/100 g). Moreover, the extracted compounds showcased significant antioxidant and antimicrobial properties.},
organization = {Ministry of Universities of the Government of Spain
Universidad de Cádiz},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
keywords = {Mulberries},
keywords = {Enzyme-assisted extraction},
keywords = {Antioxidant activity},
keywords = {Antimicrobial properties},
keywords = {Box–Behnken optimization},
keywords = {Plackett–Burman},
title = {Enhancing Efficiency of Enzymatic-Assisted Extraction Method for Evaluating Bioactive Compound Analysis in Mulberry: An Optimization Approach},
doi = {10.3390/agronomy13102548},
author = {Tizón Alba, A. and Aliaño González, María José and Palma Lovillo, Miguel and Fernández Barbero, Gerardo and Carrera Fernández, Ceferino Adrián},
}