@misc{10498/32727,
year = {2023},
url = {http://hdl.handle.net/10498/32727},
abstract = {Although cardinal cortical interneuron identity is established upon cell-cycle exit, it remains unclear whether specific interneuron subtypes are pre-established, and if so, how their identity is maintained prior to circuit integration. We conditionally removed Sox6 (Sox6-cKO) in migrating somatostatin (Sst+) interneurons and assessed the effects on their mature identity. In adolescent mice, five of eight molecular Sst+ subtypes were nearly absent in the Sox6-cKO cortex without a reduction in cell number. Sox6-cKO cells displayed electrophysiological maturity and expressed genes enriched within the broad class of Sst+ interneurons. Furthermore, we could infer subtype identity prior to cortical integration (embryonic day 18.5), suggesting that the loss in subtype was due to disrupted subtype maintenance. Conversely, Sox6 removal at postnatal day 7 did not disrupt marker expression in the mature cortex. Therefore, Sox6 is necessary during migration for maintenance of Sst+ subtype identity, indicating that subtype maintenance requires active transcriptional programs.},
publisher = {CELL PRESS},
keywords = {CELL-TYPES},
keywords = {GABAERGIC INTERNEURONS},
keywords = {SUPER-ENHANCERS},
keywords = {SKELETAL-MUSCLE},
keywords = {GENE-EXPRESSION},
keywords = {RNA-SEQ},
keywords = {SOX6},
keywords = {DIVERSITY},
keywords = {FATE},
keywords = {LAYER},
title = {Transcriptional maintenance of cortical somatostatin interneuron subtype identity during migration},
doi = {10.1016/J.NEURON.2023.07.018},
author = {Munguba, Hermany and Nikouei, H. and Hochgerner, H. and Oberst, P. and Kouznetsova, A. and Ryge, J. and Muñoz Manchado, Ana Belén and Close, J. and Batista-Brito, Renata and Linnarsson, S. and Hjerling-Leffler, J.},
}