@misc{10498/33966, year = {2017}, url = {http://hdl.handle.net/10498/33966}, abstract = {We developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obtained after conventional laboratory procedures including subcultures. In 10 samples misidentified by direct processing, yeasts or mixed cultures grew in the thioglycolate subcultures, or high cellular debris hampered a correct analysis. This rapid method can provide a fast, clinically- relevant species-level identification without disturbing the daily workflow in clinical microbiology laboratories.}, publisher = {Public Library of Science}, title = {Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS}, doi = {10.1371/JOURNAL.PONE.0185229}, author = {Arca Suárez, Jorge and Galán Sánchez, Fátima and Marín Casanova, Pilar and Rodríguez Iglesias, Manuel Antonio}, }