@misc{10498/34929,
year = {2017},
month = {1},
url = {http://hdl.handle.net/10498/34929},
abstract = {Wine industry needs a simple method for rapid diagnosis of the dominance of inoculated strains that could be performed routinely during the fermentation process. We present a suitable, high-throughput, and low-cost method to monitor rapidly the dominance of inoculated yeast strains in industrial fermentations of red and white wines using an activated carbon cleaning pretreatment, and a rapid DNA extraction method plus multiplex PCR-SSR analysis. We apply this technique directly to samples of fermenting wines without previously isolating yeast colonies. Results are obtained in a maximum time of 4.5 h.},
keywords = {Activated carbon},
keywords = {DNA extraction},
keywords = {Microsatellite},
keywords = {Fermentation control},
keywords = {Saccharomyces cerevisiae},
title = {Rapid and not culture-dependent assay based on multiplex PCR-SSR analysis for monitoring inoculated yeast strains in industrial wine fermentations.},
doi = {10.1007/s00203-016-1287-4},
author = {Cordero Bueso, Gustavo Adolfo and Rodríguez Jiménez, María Esther and Garrido Crespo, Carlos and Cantoral Fernández, Jesús Manuel},
}