@misc{10498/36279, year = {2013}, month = {10}, url = {http://hdl.handle.net/10498/36279}, abstract = {Rotifers, used in aquaculture as feed for early larval stages, are poor in carotenoids compared with cope- pods, the natural most common prey item of marine fish larvae. A minimal sufficient level of carotenoids in fish larvae may be essential for a correct develop- ment. The freshwater microalga Muriellopsis sp. has been identified as a potential source of the caroten- oid lutein with possible application in aquaculture. Lutein is a potent antioxidant, with potentially ben- eficial effects as a carotenoid for fish larvae. This study describes the lutein enrichment of the rotifer Brachionus sp. when incubated with homogeneously dispersed suspensions of freeze-dried, lutein-rich Muriellopsis sp. in seawater. A short enrichment time (2 or 2.5 h) resulted in a higher lutein content of the rotifers than a long enrichment time (13.5 or 24 h). Although the concentration of lutein in enriched rotifers, 0.06–0.16 mg (g dry weight) 1 was low compared with its content in Muriellopsis sp. [about 3 mg (g dry weight) 1], it was in the range reported for natural copepod populations. Therefore, we conclude that freeze-dried biomass of Muriellopsis sp. can be used to enrich rotifers with lutein, providing an adequate level of this caroten- oid for marine fish larvae.}, publisher = {Wiley}, keywords = {Muriellopsis sp.}, keywords = {microalga}, keywords = {lutein}, keywords = {rotifer enrichment}, keywords = {Brachionus sp.}, title = {Lutein enrichment of the rotifer Brachionus sp. using freeze-dried Muriellopsis sp. cells}, doi = {10.1111/j.1365-2109.2012.03178.x}, author = {van Bergeijk, Stefanie Anne and Paullada Salmerón, José Antonio and López-Pérez, Ana Isabel and Moreno, José and Cañavate, José Pedro and García-González, Mercedes}, }