@misc{10498/36492,
year = {2025},
url = {http://hdl.handle.net/10498/36492},
abstract = {Background: Measuring maximal fat oxidation (MFO) and maximal oxygen consumption (V_ O2max) typically requires two separate days, which can be time-consuming. This study aimed to assess the validity and reliability of a
single-day test for measuring MFO and V_ O2max in individuals with pre-diabetes/type 2 diabetes (T2DM).
Methods: Twenty adults with pre-diabetes/T2DM (10 women; [53.1   8.8] years) participated in this crosssectional study. Participants underwent a single-day MFO- V_ O2max protocol, followed by the traditional V_ O2max
test on a cycle ergometer 4–7 days later, with both assessments performed using indirect calorimetry. The singleday test commenced with the MFO test, involving 15 W per 3 min increments until the respiratory exchange rate
reached 1.0. After a 5-minute (min) rest, the V_ O2max test was conducted, starting at the last load and increasing by
15 W⋅min-1 until voluntary exhaustion. The traditional V_ O2max test involved a 15 W⋅min-1n incremental protocol
until voluntary exhaustion, followed by a verification test at 110% of the previous maximum load.
Results: No significant differences were observed between MFO-V_ O2max and traditional V_ O2max protocol in V_ O2max
values ([2 099.4   534.1] ml⋅min-1 vs. [2 119.1   548.3] ml⋅min-1, p ¼ 0.715) or heart rate ([163.2   18.2] beat/
min vs. [164.5   16.1] beat/min, p ¼ 0.485). The MFO-V_ O2max protocol test demonstrated excellent reliability
(intraclass correlation coefficient ¼ 0.957), limits of agreement ranging from 300.06 ml⋅min-1 to 306.77 ml⋅min-1,
and the standard error of measurement was 181.67 ml⋅min-1.
Conclusions: Therefore, the single-day test is a valid and reliable method for assessing both MFO and V_ O2max in
men and women with pre-diabetes/T2DM. With a mean total duration of (34   8) min, this test enhances clinical
applicability by significantly reducing the time and cost associated with traditional assessments.},
publisher = {KeAi},
keywords = {Validation},
keywords = {Cardiorespiratory fitness},
keywords = {FatMax},
keywords = {Metabolic flexibility},
keywords = {Exercise tests},
keywords = {Exercise physiology},
title = {Validity and reliability of V˙O2max determination immediately after a maximal fat oxidation assessment in pre-diabetes/type 2 Diabetes Mellitus population},
doi = {https://doi.org/10.1016/j.smhs.2025.02.004},
author = {Marín Galindo, Alberto and Opazo Díaz, Edgardo and Ramírez Jiménez, Miguel and Montes de Oca García, Adrian and Corral Pérez, Juan and Pérez Pérez, Alejandro and Casals Vázquez, Cristina and Ponce González, Jesús Gustavo},
}