@misc{10498/36665,
year = {2025},
url = {http://hdl.handle.net/10498/36665},
abstract = {The brown macroalga Rugulopteryx okamurae was used to produce a sugar-rich hydrolysate,
which served as a carbon source in Paraburkholderia sacchari DSM 17165 cultures for the production
of poly(3-hydroxybutyrate) (PHB). Biological pretreatment of R. okamurae using the
fungal strains Aspergillus awamori and Aspergillus oryzae in solid-state fermentation (SSF) was
employed to produce alginate lyase (24 IU/gbiomass) and protease (20 IU/gbiomass). The fermented
solids were used for enzymatic hydrolysis in combination with a commercial cocktail of enzymes
containing cellulase and hemicellulase. SSF of the macroalgae for 72 h by A. oryzae, followed by
enzymatic saccharification for 48 h, resulted in a total reducing sugars (TRS) concentration of
around 120 mgTRS/gbiomass. Conducting a bioreactor batch fermentation on macroalgae-derived
hydrolysate for PHB production by P. sacchari, consuming 51 g/L of glucose, resulted in
43.3 g/L of dry cell weight with 28.4 % PHB content (12.3 g/L PHB). This study demonstrated the
feasibility of exploiting macroalgae as a feedstock for PHB production.},
publisher = {Elsevier},
keywords = {Rugulopteryx okamurae},
keywords = {Solid-state fermentation},
keywords = {Enzymatic hydrolysis},
keywords = {Paraburkholderia sacchari},
keywords = {Poly(3-hydroxybutyrate)},
title = {Bioprocess development for the production of poly(3-hydroxybutyrate) using the invasive macroalgae Rugulopteryx okamurae},
doi = {https://doi.org/10.1016/j.eti.2025.104342},
author = {Agabo García, Cristina and Sarafidou, Mirva and Psaki, Olga and Argeiti, Chrysanthi and Blandino Garrido, Ana María and Álvarez Gallego, Carlos José and Romero García, Luis Isidoro and Koutinas, Apostolis},
}