@misc{10498/36670,
year = {2017},
url = {http://hdl.handle.net/10498/36670},
abstract = {Therapeutic hypothermia (TH) initiated within 6 h from birth is the most effective therapeutic approach for moderate to severe hypoxic-ischemic encephalopathy (HIE). However, underlying mechanisms and effects on the human metabolism are not yet fully understood. This work aims at studying the evolution of several energy related key metabolites in newborns with HIE undergoing TH employing gas chromatography - mass spectrometry. The method was validated following stringent FDA requirements and applied to 194 samples from a subgroup of newborns with HIE (N = 61) enrolled in a multicenter clinical trial (HYPOTOP) for the determination of lactate, pyruvate, ketone bodies and several Krebs cycle metabolites at different sampling time points. The analysis of plasma samples from newborns with HIE revealed a decrease of lactate, pyruvate and β-hydroxybutyrate concentrations, whereas rising malate concentrations were observed. In healthy control newborns (N = 19) significantly lower levels of pyruvate and lactate were found in comparison to age-matched newborns with HIE undergoing TH, whereas acetoacetate and β-hydroxybutyrate levels were clearly increased. Access to a validated analytical method and a controlled cohort of newborns with HIE undergoing hypothermia treatment for the first time allowed the in-depth study of the evolution of key metabolites of metabolic junctions in this special population.},
publisher = {Nature Publishing Group},
title = {Evolution of energy related metabolites in plasma from newborns with hypoxic-ischemic encephalopathy during hypothermia treatment},
doi = {10.1038/S41598-017-17202-7},
author = {Sánchez Illana, Ángel and Núñez Ramiro, Antonio and Cernada, María and Parra Llorca, Anna and Valverde, Eva and Blanco, Dorotea and Moral Pumarega, María Teresa and Cabañas, Fernando and Boix, Héctor and Pavón, Antonio and Chafanel, Mercedes and Benavente Fernández, Isabel and Tofe, Inés and Loureiro, Begoña and Fernández Lorenzo, José R. and Fernández Colomer, Belén and García Robles, Ana and Kuligowski, Julia and Vento, Máximo and Cordeiro, M. and Arriaga, M. and Ureta Velasco, N. and Caballero, M.A. and Fernández, C. and Castilla, Y. and Ferreira, J.F. and Lubián López, Simón Pedro and Jaraba, P. and López Heredia, J.},
}