@misc{10498/36768,
year = {2025},
url = {http://hdl.handle.net/10498/36768},
abstract = {The aim of this study was to investigate the evolution of the microbial community and its relationship with hydrogen
production, as well as pathogen reduction during thermophilic dark fermentation using sewage sludge
and wine vinasses, under ozone pre-treatment (PT) and non-pre-treated (Control) conditions. Microbial dynamics
analysis revealed that PT increased the Eubacteria ratio and shifted the balance between Hydrolytic-
Acidogenic Bacteria (HABs) and acetogens. PT enhanced bio-hydrogen production, achieving a 78 % increase in
yield and a 90 % improvement in specific hydrogen production compared to control. In both conditions, Eubacteria
predominated over Archaea, with a higher proportion of Propionate-Utilizing Acetogens in comparison with
Butyrate-Utilizing Acetogens. Hydrolytic activity was 25 % higher in PT, correlating with the highest hydrogen
production, reduced non-soluble carbon, and the most active HABs. PT also increased acidification yields by
22 % and microbial activity by 80 %, enhancing hydrogen production primarily through elevated hydrolytic activity.
This was associated with a 60 % rise in acid carbon, with acetic and butyric acids comprising 80 % of the
total volatile fatty acids. Regarding pathogen reduction, PT reduced Escherichia coli concentrations in the feed by
an order of magnitude. In conclusion, PT significantly improved bio-hydrogen production, microbial activity,
and process efficiency, while facilitating effective pathogen reduction.},
publisher = {Elsevier},
keywords = {Bio-hidrógeno},
keywords = {Ácidos Grasos Volátiles},
keywords = {Ozono},
keywords = {Fermentación Oscura},
keywords = {Microbiota},
keywords = {Patógenos},
keywords = {Hydrogen production},
keywords = {Population dynamics},
keywords = {Hydrolytic-acidogenic bacteria},
keywords = {Hydrolysis yield},
keywords = {Acidic carbon},
title = {Effect of an ozone pre-treatment on dark fermentation: Influence on the microbiota and pathogen removal},
doi = {https://doi.org/10.1016/j.jenvman.2025.126330},
author = {Ibáñez López, María Eugenia and Díaz Domínguez, Encarnación and García Morales, José Luis},
}