@misc{10498/39326,
year = {2025},
month = {11},
url = {http://hdl.handle.net/10498/39326},
abstract = {Biorefinery is an innovative concept for resource utilization. To further increase its efficiency, the integration of different biomass conversion technologies (integrated biorefinery) is essential. Biodiesel, a traditional biofuel, generates glycerol as an inevitable byproduct; making it an attractive carbon source for biorefineries due to its abundance, low price, and high degree of reduction. In this work, we propose an integrated two-step process of dark fermentation with Escherichia coli to produce L-malate from glycerol and photofermentation by Rhodobacter capsulatus to convert this L-malate into hydrogen, a clean and sustainable energy carrier. To this end, we optimized an E. coli L-malate-producing strain by overexpressing GlpK in the M4-Δiclr/pBAD-pck strain and scale up the process from shake flask to bioreactor using waste crude glycerol from a biodiesel biorefinery instead of pure glycerol. Under the optimized conditions, E. coli produced up to 11 g/L L-malate in 24 h. The culture medium from this dark fermentation was used to formulate an L-malate enriched medium for Rhodobacter that led to the production of 58.0 ± 6 mM H2 in 90 h.},
publisher = {BioMed Central Ltd},
keywords = {Biorefinery},
keywords = {Biodiesel},
keywords = {Crude glycerol},
keywords = {L-malate},
keywords = {Bio-H2},
keywords = {Escherichia coli},
keywords = {Rhodobacter capsulatus},
title = {Production of hydrogen from crude glycerol via an integrated process of L-malate biosynthesis by Escherichia coli and photofermentation by Rhodobacter capsulatus},
doi = {10.1186/S12934-025-02866-Y},
author = {Marcos Sánchez, José Luis and Cabrera Revuelta, Gema and Hernandez, Daniel  F. and Luque, Beatriz and Valle Gallardo, Antonio and Bolívar Pérez, Jorge},
}