%0 Journal Article 
%A Callejo-Lopez, J.A.
%A Ramírez Muñoz, Martín
%A González Cortés, José Joaquín
%A Valle Gallardo, Antonio
%A Cantero Moreno, Domingo
%A Bolívar Pérez, Jorge
%T Application of a Microalgal Peptide-Enriched Extract as Media Component in E. coli Culture
%D 2023
%@ 3529-3538
%U http://hdl.handle.net/10498/28399
%X This study evaluated the feasibility of applying a microalgal peptide-enriched extract, obtained through a sequential alkaline and enzymatic method, as a fundamental media component in Escherichia coli culture. The results demonstrated that the extract is viable as the primary nutrient source for E. coli cultures (wild type), even as a complete substitute for the widely used Luria-Bertani (LB) medium, using an extract dilution of 1:3. Its application in a proportion carbon limiting (dilution 1:7), with glycerol supplementation at the early stationary phase, improved the nutrient use efficiency and controlled the undesirable acetate production. Furthermore, this last strategy applied in an E. coli mutant (M4-Delta iclR) enhanced the production of succinate by 67.3% compared with the M9 medium. The innovative approach proposed in this study opens a wide range of applications and studies related to the production of bio-compounds through diverse microorganisms.
%K microalgae
%K peptide-enriched extract
%K culture medium
%K E. coli
%K succinate
%~ Universidad de Cádiz