%0 Journal Article 
%A Corrêa de Souza, Mariana
%A de Souza Mesquita, Leonardo M.
%A Pena, Fabíola L.
%A Tamborlin, Leticia
%A Capelasso da Silva, Laise
%A Viganó, Juliane
%A Costa Antunes, Adriane E
%A Luchessi, Augusto D.
%A Teixeira Duarte, Marta C.
%A Fernández Barbero, Gerardo
%A Ariel Rostagno, Mauricio
%T Potential application for antimicrobial and antileukemic therapy of a flavonoid-rich fraction of Camellia sinensis
%D 2022
%@ 2772-753X
%U http://hdl.handle.net/10498/29445
%X The antimicrobial and antileukemic effect of a purified fraction of flavonoids from the leaves of Camellia sinensis was evaluated. An extraction yield of 9.77 mg.g-1 total flavonoids was recovered through a pressurized liquid extraction associated with solid-phase extraction. This fraction was tested against pathogenic microorganisms (Staphylococcus, Salmonella, and Enterococcus), considering the minimum inhibitory concentration. In addition, the human monocyte cell line THP-1, derived from a patient with acute monocytic leukemia, was used for the antitumor assay. The results show that the flavonoid-rich fraction obtained by coupling a Pressurized Liquids Extraction in-line with a Solid Phase Extraction (PLE-SPE) has a high antimicrobial effect and resulted in cell cycle blockage G0 / G1, increased DNA fragmentation, and altered leukemic cell morphology. These results suggest that a flavonoid-rich fraction obtained from Camellia sinensis can be applied as potential adjuvants in chemotherapy treatment to mitigate the side effects caused by chemotherapy or even as a supplement to cancer therapy
%K Coupled extraction techniques
%K Flavonoids
%K Theaflavin
%K Cancer therapy
%K THP-1
%~ Universidad de Cádiz