%0 Journal Article 
%A Espada Bellido, Estrella
%A Ferreiro González, Marta
%A Carrera Fernández, Ceferino Adrián
%A Palma Lovillo, Miguel
%A García Barroso, Carmelo
%A Fernández Barbero, Gerardo
%T Optimization of the ultrasound-assisted extraction of anthocyanins and total phenolic compounds in mulberry (Morus nigra) pulp
%D 2017
%@ 1873-7072

%U http://hdl.handle.net/10498/30062
%X New ultrasound-assisted extraction methods for the determination of anthocyanins and total phenolic
compounds present in mulberries have been developed. Several extraction variables, including methanol
composition (50–100%), temperature (10–70  C), ultrasound amplitude (30–70%), cycle (0.2–0.7 s),
solvent pH (3–7) and solvent-solid ratio (10:1.5–20:1.5) were optimized. A Box–Behnken design in
conjunction with a response surface methodology was employed to optimize the conditions for the
maximum response based on 54 different experiments. Two response variables were considered: total
anthocyanins and total phenolic compounds. Extraction temperature and solvent composition were
found to be the most influential parameters for anthocyanins (48  C and 76%) and phenolic compounds
(64  C and 61%). The developed methods showed high reproducibility and repeatability (RSD < 5%).
Finally, the new methods were successfully applied to real samples in order to investigate the presence
of anthocyanins and total phenolic compounds in several mulberry jams.
%K Anthocyanins
%K Box–Behnken design
%K Mulberry
%K Phenolic compounds
%K Ultrasound-assisted extraction
%~ Universidad de Cádiz