%0 Journal Article 
%A Olvera Collantes, Lucía
%A Moares Fernández, Noelia del Carmen
%A Fernández Cisnal, R.
%A Muñoz Miranda, Juan Pedro
%A González García, Pablo
%A Gabucio López, Antonio
%A Freyre Carrillo, Carolina
%A Jordan-Chaves, Juan de Dios
%A Trujillo-Soto, Teresa
%A Rodriguez-Martinez, María P.
%A Martin-Rubio, Maria I.
%A Escuer, Eva
%A Rodríguez Iglesias, Manuel Antonio
%A Fernández Ponce, Cecilia Matilde
%A García Cózar, Francisco José
%T Immunoassay for SARS-CoV-2 Humoral Response Monitorization: A Study of the Antibody Response in COVID-19 Patients with Different Clinical Profiles during the First and Second Waves in Cadiz, Spain
%D 2023
%@ 2076-2607
%U http://hdl.handle.net/10498/30371
%X There is still a long way ahead regarding the COVID-19 pandemic, since emerging waves
remain a daunting challenge to the healthcare system. For this reason, the development of new
preventive tools and therapeutic strategies to deal with the disease have been necessary, among
which serological assays have played a key role in the control of COVID-19 outbreaks and vaccine
development. Here, we have developed and evaluated an immunoassay capable of simultaneously
detecting multiple IgG antibodies against different SARS-CoV-2 antigens through the use of Bio-
PlexTM technology. Additionally, we have analyzed the antibody response in COVID-19 patients with
different clinical profiles in Cadiz, Spain. The multiplex immunoassay presented is a high-throughput
and robust immune response monitoring tool capable of concurrently detecting anti-S1, anti-NC
and anti-RBD IgG antibodies in serum with a very high sensitivity (94.34–97.96%) and specificity
(91.84–100%). Therefore, the immunoassay proposed herein may be a useful monitoring tool for
individual humoral immunity against SARS-CoV-2, as well as for epidemiological surveillance.
In addition, we show the values of antibodies against multiple SARS-CoV-2 antigens and their
correlation with the different clinical profiles of unvaccinated COVID-19 patients in Cadiz, Spain,
during the first and second waves of the pandemic.
%K SARS-CoV-2
%K COVID-19
%K antibody response
%K IgG antibodies
%K multiplex immunoassay
%K monitoring tool
%~ Universidad de Cádiz