%0 Journal Article 
%A Cuesta Sancho, Sara
%A Márquez Ruiz, Denisse
%A Illanes Álvarez, Francisco
%A Campaña Gómez, Irene
%A Martín Aspas, Andrés
%A Trujillo Soto, María Teresa
%A Romero, Alberto
%A Galán Sánchez, Fátima
%A Rodríguez Iglesias, Manuel Antonio
%A Márquez Coello, María de las Mercedes
%A Girón González, José Antonio
%T Expression profile of microRNAs related with viral infectivity, inflammatory response, and immune activation in people living with HIV
%D 2023
%@ 1664-302X
%U http://hdl.handle.net/10498/31173
%X Objective: To evaluate the serum expression of microRNAs (miRNAs) with ability to modulate the human immunodeficiency (HIV) replication or inflammatory status in people living with HIV (PLWH). Methods: Forty healthy controls and two groups of PLWH were evaluated: (a) Group 1 (n = 30), patients with detectable viral load at inclusion, analyzed before receiving antiretroviral therapy (ART) and 12 months after initiating it; (b) Group 2 (n = 55), PLWH with prolonged undetectable viral load. Intestinal barrier disruption (I-FABP) and bacterial translocation (16S rDNA) markers, inflammatory markers such as interleukin (IL)-6 and sCD163, immune activation and expression of specific miRNAs were evaluated. Results: Serum concentrations of I-FABP, 16S rDNA, IL-6, sCD163 and activated T lymphocytes were increased in PLWH. Serum miR-34a was overexpressed at inclusion and remained elevated after ART. The expression of the remaining miRNAs that modulate HIV infectivity (miR-7, mir-29a, miR-150, and miR-223) was similar in PLWH and controls. Related to miRNAs implicated in inflammation (miR-21, miR-155, and miR-210), significant overexpression were observed in miR-21 and miR-210 levels in untreated PLWH, but levels were restored in those patients treated for a long period. Conclusion: A sustained overexpression of miR-34a was detected even after prolonged HIV controlled replication. miR-21 and miR-210 can be considered new markers of inflammation with high sensitivity to its modifications.
%K bacterial translocation
%K Human Immunodeficiency (HIV)
%K Intestinal Barrier Disruption (I-FABP)
%K interleukin (IL)-6
%K sCD163
%K miRNAs
%K people living with HIV
%~ Universidad de Cádiz