%0 Journal Article 
%A Tizón Alba, A.
%A Aliaño González, María José
%A Palma Lovillo, Miguel
%A Fernández Barbero, Gerardo
%A Carrera Fernández, Ceferino Adrián
%T Enhancing Efficiency of Enzymatic-Assisted Extraction Method for Evaluating Bioactive Compound Analysis in Mulberry: An Optimization Approach
%D 2023
%@ 2073-4395
%U http://hdl.handle.net/10498/31822
%X The present investigation endeavors to optimize a method based on enzyme-assisted extraction for the efficient retrieval of bioactive compounds from mulberry, leveraging its notable health-promoting properties. A combined approach of Plackett–Burman design followed by Box–Behnken design was employed for determining the crucial extraction parameters and subsequently, refining the process. Optimal conditions consisted of heating 0.15 g of mulberry at 40 °C, using 15 mL of 70% EtOH as a solvent at pH 4, 38.46 enzyme units per g of sample, and shaking at 200 rpm. The optimum extraction time study revealed that 5 min of extraction was sufficient to reach the maximum concentration of the bioactive compound. The repeatability and intermediate precision assessment exhibited a coefficient of variation below 5%. Among the diverse mulberry varieties scrutinized, Morus nigra showed the highest anthocyanin content (27.90 ± 2.14 mg/100 g), while Morus rubra showed the highest concentration of phenolic compounds (121.10 ± 19.56 mg/100 g). Moreover, the extracted compounds showcased significant antioxidant and antimicrobial properties.
%K Mulberries
%K Enzyme-assisted extraction
%K Antioxidant activity
%K Antimicrobial properties
%K Box–Behnken optimization
%K Plackett–Burman
%~ Universidad de Cádiz