%0 Journal Article 
%A Munguba, Hermany
%A Nikouei, H.
%A Hochgerner, H.
%A Oberst, P.
%A Kouznetsova, A.
%A Ryge, J.
%A Muñoz Manchado, Ana Belén
%A Close, J.
%A Batista-Brito, Renata
%A Linnarsson, S.
%A Hjerling-Leffler, J.
%T Transcriptional maintenance of cortical somatostatin interneuron subtype identity during migration
%D 2023
%@ 1097-4199

%U http://hdl.handle.net/10498/32727
%X Although cardinal cortical interneuron identity is established upon cell-cycle exit, it remains unclear whether specific interneuron subtypes are pre-established, and if so, how their identity is maintained prior to circuit integration. We conditionally removed Sox6 (Sox6-cKO) in migrating somatostatin (Sst+) interneurons and assessed the effects on their mature identity. In adolescent mice, five of eight molecular Sst+ subtypes were nearly absent in the Sox6-cKO cortex without a reduction in cell number. Sox6-cKO cells displayed electrophysiological maturity and expressed genes enriched within the broad class of Sst+ interneurons. Furthermore, we could infer subtype identity prior to cortical integration (embryonic day 18.5), suggesting that the loss in subtype was due to disrupted subtype maintenance. Conversely, Sox6 removal at postnatal day 7 did not disrupt marker expression in the mature cortex. Therefore, Sox6 is necessary during migration for maintenance of Sst+ subtype identity, indicating that subtype maintenance requires active transcriptional programs.
%K CELL-TYPES
%K GABAERGIC INTERNEURONS
%K SUPER-ENHANCERS
%K SKELETAL-MUSCLE
%K GENE-EXPRESSION
%K RNA-SEQ
%K SOX6
%K DIVERSITY
%K FATE
%K LAYER
%~ Universidad de Cádiz