%0 Journal Article 
%A Muñoz Manchado, Ana Belen
%A Bengtsson Gonzales, C.
%A Zeisel, A.
%A Munguba, H.
%A Bekkouche, B.
%A Skene, N.G.
%A Lönnerberg, P.
%A Ryge, J.
%A Harris, K.D.
%A Linnarsson, S.
%A Hjerling-Leffler, Jens
%T Diversity of Interneurons in the Dorsal Striatum Revealed by Single-Cell RNA Sequencing and PatchSeq
%D 2018
%@ 2211-1247
%U http://hdl.handle.net/10498/33429
%X Striatal locally projecting neurons, or interneurons, act on nearby circuits and shape functional output to the rest of the basal ganglia. We performed sin- gle-cell RNA sequencing of striatal cells enriching for interneurons. We find seven discrete interneuron types, six of which are GABAergic. In addition to providing specific markers for the populations previ- ously described, including those expressing Sst/Npy, Th, Npy without Sst, and Chat, we identify two small populations of cells expressing Cck with or without Vip. Surprisingly, the Pvalb-expressing cells do not constitute a discrete cluster but rather are part of a larger group of cells expressing Pthlh with a spatial gradient of Pvalb expression. Using PatchSeq, we show that Pthlh cells exhibit a continuum of electro- physiological properties correlated with expression of Pvalb. Furthermore, we find significant molecular differences that correlate with differences in electro- physiological properties between Pvalb-expressing cells of the striatum and those of the cortex.
%K Pthlh
%K cell classes
%K cortex
%K electrophysiology
%K fast-spiking neurons
%K interneuron
%K parvalbumin
%K single-cell RNA sequencing
%K striatum
%~ Universidad de Cádiz