%0 Journal Article 
%A Fernandez, R.
%A Colás Ruiz, Nieves del Rocio
%A Lara Martín, Pablo Antonio
%A Fernández Cisnal, R.
%A Hampel, Miriam
%A Hampel
%T Proteomic analysis in the brain and liver of sea bream (Sparus aurata) exposed to the antibiotics ciprofloxacin, sulfadiazine, and trimethoprim
%D 2024
%@ 1873-6424

%U http://hdl.handle.net/10498/33640
%X Antibiotics, frequently detected in aquatic ecosystems, can negatively impact the health of resident organisms.
Although the study on the possible effects of antibiotics on these organisms has been increasing, there is still little
information available on the molecular effects on exposed non-target organisms. In our study we used a label free
proteomic approach and sea bream, Sparus aurata, to evaluate the effects of exposure to environmentally relevant
concentrations of the antibiotic compounds ciprofloxacin (CIP), sulfadiazine (SULF) and trimethoprim (TRIM)
produced at the protein level. Individuals of sea bream were exposed to single compounds at 5.2 ± 2.1 μg L− 1 of
CIP, 3.8 ± 2.7 μg L− 1 of SULF and 25.7 ± 10.8 μg L− 1 of TRIM for 21 days. After exposure, the number of
differentially expressed proteins in the liver was 39, 73 and 4 for CIP, SULF and TRIM respectively. In the brain,
there was no alteration of proteins after CIP and TRIM treatment, while 9 proteins were impacted after SULF
treatment. The differentially expressed proteins were involved in cellular biological, metabolic, developmental,
growth and biological regulatory processes. Overall, our study evidences the vulnerability of Sparus aurata, after
exposure to environmentally relevant concentrations of the major antibiotics CIP, SULF and TRIM and that their
chronic exposure could lead to a stress situation, altering the proteomic profile of key organs such as brain and
liver.
%K Antibiotics
%K Proteomics
%K Sparus aurata
%K Emerging pollutants
%~ Universidad de Cádiz