%0 Journal Article 
%A Cordero Bueso, Gustavo Adolfo
%A Rodríguez Jiménez, María Esther
%A Garrido Crespo, Carlos
%A Cantoral Fernández, Jesús Manuel
%T Rapid and not culture-dependent assay based on multiplex PCR-SSR analysis for monitoring inoculated yeast strains in industrial wine fermentations.
%D 2017
%@ 1432-072X,

%U http://hdl.handle.net/10498/34929
%X Wine industry needs a simple method for rapid diagnosis of the dominance of inoculated strains that could be performed routinely during the fermentation process. We present a suitable, high-throughput, and low-cost method to monitor rapidly the dominance of inoculated yeast strains in industrial fermentations of red and white wines using an activated carbon cleaning pretreatment, and a rapid DNA extraction method plus multiplex PCR-SSR analysis. We apply this technique directly to samples of fermenting wines without previously isolating yeast colonies. Results are obtained in a maximum time of 4.5 h.
%K Activated carbon
%K DNA extraction
%K Microsatellite
%K Fermentation control
%K Saccharomyces cerevisiae
%~ Universidad de Cádiz