%0 Journal Article 
%A Blanco-Andújar, Cristina
%A Ortega Ponce, Daniel
%A Southern, Paul
%A Steven, Nesbitt
%A Thanh, Nguyen Thi Kim
%A Pankhurst, Quentin Andrew
%T Real-time tracking of delayed-onset cellular apoptosis induced by intracellular magnetic hyperthermia
%D 2015
%@ 1748-6963

%U http://hdl.handle.net/10498/35264
%X Aim: To assess cell death pathways in response to magnetic hyperthermia. Materials & methods: Human melanoma cells were loaded with citric acid-coated iron-oxide nanoparticles, and subjected to a time-varying magnetic field. Pathways were monitored in vitro in suspensions and in situ in monolayers using fluorophores to report on early-stage apoptosis and late-stage apoptosis and/or necrosis. Results: Delayed-onset effects were observed, with a rate and extent proportional to the thermal-load-per-cell. At moderate loads, membranal internal-to-external lipid exchange preceded rupture and death by a few hours (the timeline varying cell-to-cell), without any measurable change in the local environment temperature. Conclusion: Our observations support the proposition that intracellular heating may be a viable, controllable and nonaggressive in vivo treatment for human pathological conditions.
%K apoptosis
%K magnetic hyperthermia
%K in vitro
%K melanoma
%~ Universidad de Cádiz