%0 Journal Article %A van Bergeijk, Stefanie Anne %A Paullada Salmerón, José Antonio %A López-Pérez, Ana Isabel %A Moreno, José %A Cañavate, José Pedro %A García-González, Mercedes %T Lutein enrichment of the rotifer Brachionus sp. using freeze-dried Muriellopsis sp. cells %D 2013 %@ 13652109 %U http://hdl.handle.net/10498/36279 %X Rotifers, used in aquaculture as feed for early larval stages, are poor in carotenoids compared with cope- pods, the natural most common prey item of marine fish larvae. A minimal sufficient level of carotenoids in fish larvae may be essential for a correct develop- ment. The freshwater microalga Muriellopsis sp. has been identified as a potential source of the caroten- oid lutein with possible application in aquaculture. Lutein is a potent antioxidant, with potentially ben- eficial effects as a carotenoid for fish larvae. This study describes the lutein enrichment of the rotifer Brachionus sp. when incubated with homogeneously dispersed suspensions of freeze-dried, lutein-rich Muriellopsis sp. in seawater. A short enrichment time (2 or 2.5 h) resulted in a higher lutein content of the rotifers than a long enrichment time (13.5 or 24 h). Although the concentration of lutein in enriched rotifers, 0.06–0.16 mg (g dry weight) 1 was low compared with its content in Muriellopsis sp. [about 3 mg (g dry weight) 1], it was in the range reported for natural copepod populations. Therefore, we conclude that freeze-dried biomass of Muriellopsis sp. can be used to enrich rotifers with lutein, providing an adequate level of this caroten- oid for marine fish larvae. %K Muriellopsis sp. %K microalga %K lutein %K rotifer enrichment %K Brachionus sp. %~ Universidad de Cádiz