%0 Journal Article 
%A Ruiz Muñoz, Marina
%A Bernal Grande, María del Carmen
%A Cordero Bueso, Gustavo Adolfo
%A González, Mónica
%A Hughes Herrera, David
%A Cantoral Fernández, Jesús Manuel
%T A microtiter plate assay as a reliable method to assure the identification and classification of the veil-forming yeasts during Sherry wines ageing
%D 2017
%@ 2311-5637
%U http://hdl.handle.net/10498/36511
%X Yeasts involved in veil formation during biological ageing of Sherry wines are mainly
Saccharomyces cerevisiae, and they have traditionally been divided into four races or varieties:
beticus, cheresiensis, montuliensis and rouxii. Recent progress in molecular biology has led to
the development of several techniques for yeast identification, based on similarity or dissimilarity
of DNA, RNA or proteins. In view of the latest yeast taxonomy, there are no more races. However,
molecular techniques are not enough to understand the real veil-forming yeast diversity and dynamics
in Sherry wines. We propose a reliable method, using a microtiter reader, to evaluate the fermentation
and assimilation of carbon and nitrogen sources, the osmotolerance and the antibiotic resistance,
using 18 S. cerevisiae and 5 non-Saccharomyces yeast strains, to allow correct identification and
classification of the yeast strains present in the velum of flor complex.
%K flor yeast
%K microplate
%K fermentation
%K assimilation
%K Saccharomyces cerevisiae
%K non-Saccharomyces
%K velum of flor
%~ Universidad de Cádiz