%0 Journal Article 
%A Valor, Luis M.
%A Rodrguez Bayona, Beatriz
%A Ramos Amaya, Ana B.
%A Brieva, Jose A.
%A Campos Caro, Antonio
%T The transcriptional profiling of human in vivo-generated plasma cells identifies selective imbalances in monoclonal gammopathies
%D 2017
%@ 1932-6203
%U http://hdl.handle.net/10498/36575
%X Plasma cells (PC) represent the heterogeneous final stage of the B cells (BC) differentiation process. To characterize the transition of BC into PC, transcriptomes from human naïve BC were compared tothose of three functionally-different subsets of human in vivo-generated PC: i) tonsil PC, mainly consisting of early PC; ii) PC released to the blood after a potent booster-immunization (mostly cycling plasmablasts); and, iii) bone marrow CD138 + PCthat represent highly mature PC and include the long-lived PC compartment. This transcriptional transition involves subsets of genes related to key processes for PC maturation: the already knownprotein processing, apoptosis and homeostasis, and of new discovery including histones, macromolecule assembly, zinc-finger transcription factors and neuromodulation. This humanPCsignature is partially reproduced in vitro and is conserved in mouse. Moreover, the present study identifies genes that define PC subtypes (e.g., proliferation-associated genes for circulating PC and transcriptional-related genes for tonsil and bone marrow PC)andproposes someputative transcriptional regulators of the human PC signatures (e.g., OCT/POU, XBP1/CREB,E2F, amongothers). Finally, we also identified a restricted imbalance of the present PC transcriptional program in monoclonal gammopathies that correlated with PC malignancy.
%~ Universidad de Cádiz