%0 Journal Article 
%A Agabo García, Cristina
%A Sarafidou, Mirva
%A Psaki, Olga
%A Argeiti, Chrysanthi
%A Blandino Garrido, Ana María
%A Álvarez Gallego, Carlos José
%A Romero García, Luis Isidoro
%A Koutinas, Apostolis
%T Bioprocess development for the production of poly(3-hydroxybutyrate) using the invasive macroalgae Rugulopteryx okamurae
%D 2025
%@ 2352-1864
%U http://hdl.handle.net/10498/36665
%X The brown macroalga Rugulopteryx okamurae was used to produce a sugar-rich hydrolysate,
which served as a carbon source in Paraburkholderia sacchari DSM 17165 cultures for the production
of poly(3-hydroxybutyrate) (PHB). Biological pretreatment of R. okamurae using the
fungal strains Aspergillus awamori and Aspergillus oryzae in solid-state fermentation (SSF) was
employed to produce alginate lyase (24 IU/gbiomass) and protease (20 IU/gbiomass). The fermented
solids were used for enzymatic hydrolysis in combination with a commercial cocktail of enzymes
containing cellulase and hemicellulase. SSF of the macroalgae for 72 h by A. oryzae, followed by
enzymatic saccharification for 48 h, resulted in a total reducing sugars (TRS) concentration of
around 120 mgTRS/gbiomass. Conducting a bioreactor batch fermentation on macroalgae-derived
hydrolysate for PHB production by P. sacchari, consuming 51 g/L of glucose, resulted in
43.3 g/L of dry cell weight with 28.4 % PHB content (12.3 g/L PHB). This study demonstrated the
feasibility of exploiting macroalgae as a feedstock for PHB production.
%K Rugulopteryx okamurae
%K Solid-state fermentation
%K Enzymatic hydrolysis
%K Paraburkholderia sacchari
%K Poly(3-hydroxybutyrate)
%~ Universidad de Cádiz