%0 Journal Article 
%A Aguilar, Silvio
%A Rosado, Daniel
%A Moreno Andrés, Javier
%A Cartuche, Luis
%A Cruz, Darío
%A Acevedo Merino, Asunción
%A Nebot Sanz, Enrique
%T Inactivation of a wild isolated Klebsiella pneumoniae by photo-chemical processes: UV-C, UV-C/H2O2 and UV-C/H2O2/Fe3+
%D 2017
%@ 1873-4308
%U http://hdl.handle.net/10498/37790
%X Shrimp (Litopenaeus vannamei) farming is an important economic activity in several countries. Pathogens in
shrimp farms and its e uents pose a potential hazard for both humans and shrimps. Wild strains of bacteria were
characterized in a shrimp farm, and Klebsiella pneumoniae was chosen as a good indicator due to its presence in
the pond and the e uent and its resistance to antibiotics. Di erent photochemical processes (UV/H2O2, UV/
H2O2/Fe3+) were tested for inactivation of wild isolated K. pneumoniae and compared to UV-C radiation. By
kinetic modelling, a kmax equal to 0.43 s−1 was obtained for UV-C treatment. After optimizing the cited pro-
cesses, ranging [H2O2]: 10–30 mg·l−1; an optimal [H2O2] of 10 mg·l−1 was found, increasing kmax on 13.63%
compared to UV-C. This optimal concentration was tested for UV/H2O2/Fe3+ process; ranging [Fe3+]: 2–20
mg·l−1. The highest yield was obtained by a [H2O2]:[Fe3+] = 10:2, which leads to 4-Log reduction in 12.88 s of
treatment. Moreover, resistance of K. pneumoniae was compared to Escherichia coli. The latter proved to be more
sensitive despite its similar cellular structure. Results suggested that the photochemical processes could enhance
disinfection e ciency, especially for photo-assisted Fenton-like process in most resistant bacteria.
%K Aquaculture
%K Iron
%K Antibiotic resistance bacteria
%K Fenton process
%K Advanced oxidation processes
%K Hydrogen peroxide
%~ Universidad de Cádiz