%0 Journal Article 
%A Putri, Anggita Rosiana
%A Setyaningsih, Widiastuti
%A Palma Lovillo, Miguel
%A Carrera Fernández, Ceferino Adrián
%A Rohman, Abdul
%A Riyanto, Sugeng
%A Jang, Young Pyo
%T Ultrasound-Assisted Extraction of Patin Fish Oil: Optimization Using Response Surface Methodology – Box Behnken Design
%D 2025
%@ 2460-1578

%U http://hdl.handle.net/10498/39305
%X The oil extracted from Pangasius micronemus, commonly known as patin
fish, is highly regarded for its omega-3 and omega-6 fatty acids content, which offer
numerous health benefits. Producing high-quality patin fish oil (PFO) rich in these
essential fatty acids requires an optimized extraction process. This study utilized
ultrasound-assisted extraction (UAE) combined with response surface methodology – Box
Behnken Design (RSM-BBD) to optimize the extraction of PFO. Five variables were
examined: temperature (30–60 °C), solvent composition (n-hexane in isopropanol, 30–
90%), amplitude (30–90%), solvent-to-sample ratio (10:1–20:1), and cycle duration (0.2–
0.8 s−1). The analysis identified solvent composition, amplitude, and solvent-to-sample
ratio as significant factors influencing the response value (p < 0.005). Optimal UAE
conditions were achieved at a temperature of 59 °C, solvent composition of 42%,
amplitude of 41%, solvent-to-sample ratio of 20:1, cycle duration of 0.8 s−1, and an
extraction time of 25 min. Fatty acid profiling revealed that PFO extracted using UAE
contained omega-3 fatty acids, including α-linolenic acid (ALA) at 1.36% and
eicosapentaenoic acid (EPA) at 0.46%, as well as omega-6 fatty acids, namely linoleic acid
(LA) at 19.06% and arachidonic acid (AA) at 0.85%. These results demonstrate the
efficiency of UAE in extracting high-quality PFO.
%K PFO
%K optimization
%K ultrasound-assisted extraction
%K esponse surface methodology
%K Box-Behnken design
%~ Universidad de Cádiz