Articulos Científicos Biomedicinahttp://hdl.handle.net/10498/66372026-11-06T10:45:36Z2026-11-06T10:45:36ZStudy of grapevine endophytes’ interaction with the host and their potential as biocontrol agents as sustainable alternative to agrochemicalsPizzi, SimonaDi Canito, AlessandraPalencia Mulero, ElenaDe Oliveira Nobre Bezerra, Carmen CrisRicciardi, ValentinaMaddalena, GiulianaMaluleke, EvelynCarmine Foschino, RobertoToffolatti, Silvia LauraFracassetti, DanielaDe Lorenzis, GabriellaCordero Bueso, Gustavo AdolfoSetati, Mathabatha EvodiaVigentini, Ileanahttp://hdl.handle.net/10498/405412026-11-05T05:05:29Z2026-01-01T00:00:00ZStudy of grapevine endophytes’ interaction with the host and their potential as biocontrol agents as sustainable alternative to agrochemicals
Pizzi, Simona; Di Canito, Alessandra; Palencia Mulero, Elena; De Oliveira Nobre Bezerra, Carmen Cris; Ricciardi, Valentina; Maddalena, Giuliana; Maluleke, Evelyn; Carmine Foschino, Roberto; Toffolatti, Silvia Laura; Fracassetti, Daniela; De Lorenzis, Gabriella; Cordero Bueso, Gustavo Adolfo; Setati, Mathabatha Evodia; Vigentini, Ileana
In sustainable vineyard management, biocontrol agents (BCAs) offer a promising alternative to chemical pesticides. This study focuses on characterizing endophytes isolated from grapevines in different regions of northern Italy for (i) their effectiveness against Botrytis cinerea through in vitro tests and (ii) their capability to survive in the presence of copper and other commercial fungicides with the final goal of investigating their possible future application for an integrated approach in vineyard. Aureobasidium pullulans strains ED203, ED206, ED217, and ED221 demonstrated significant antagonistic activity toward B. cinerea, while Bacillus velezensis ED163 showed the highest levels of pathogen inhibition. Volatile organic compounds (VOCs) analysis identified three distinct microbial clusters based on BCAs volatile profiles, highlighting the production of antifungal compounds such as alcohols, esters, and terpenes to counteract the pathogen growth. The investigated BCAs showed tolerance to high concentrations of copper (up to 100 mg/L) and the fungicide SWITCH (1 g/L), but they were sensitive to a standard oenological dose of sulfur dioxide (50 mg/L). Finally, in vivo tests on grapevine leaves confirmed the ability of B. velezensis and A. pullulans strains ED203 and ED221 candidates to effectively reduce the mycelium infection on plant tissue standing out as strong candidates for pest management. The interaction between BCAs with the host was confirmed through leaves cell culture use, highlighting the capability of the best candidate selected in the study to modulate plant response in presence of pathogen.
2026-01-01T00:00:00ZA matched case-control study on Escherichia coli factors contributing to sepsis and septic shock in bacteraemic patientsMaldonado, NataliaLópez-Hernández, InmacualdaValik, John KarlssonLópez Cortés, Luis EduardoMartínez Pérez-Crespo, Pedro MaríaGarcia-Montaner, AndreaAlcalde-Rico, ManuelSousa Dominguez, AdriánJover Sáenz, AlfredoGoikoetxea Aguirre, Ane JosunePulido Navazo, ÁngelesBuzon-Martin, LuisAller, Ana IsabelBoix Palop, Lucíadel Arco Jiménez, AlfonsoSmithson Amat, AlejandroSánchez Calvo, Juan ManuelNatera Kindelán, ClaraReguera Iglesias, José MaríaArmiñanzas Castillo, CarlosGalán Sánchez, FátimaBahamonde Carrasco, AlbertoGea Lázaro, IsabelCastello-Abietar, CristianPérez Camacho, InésMarrodán Ciordia, TeresaBecerril-Carral, BertaNaucler, PontusPascual-Hernandez, ÁlvaroRodríguez Baño, JesúsLópez Hernández, InmaculadaRetamar Gentil, PilarPascual, ÁlvaroLanz García, JoaquínLeón Jiménez, EvaCorzo-Delgado, Juan EnriquePérez Rodríguez, María TeresaBarcenilla Gaite, FernandoMontejo Baranda, MiguelCuquet Pedragosa, JordiLabayru Echeverria, CristinaMantecon Vallejo, María ÁngelesBoix Palop, LucíaCalbo Sebastian, EstherFarinas Alvarez, María Del CarmenFernández Suárez, JonathanArias Temprano, MartaCanueto Quintero, JesúsReyes Bertos, ArmandoSánchez Porto, AntonioVinuesa García, DavidGuzmán García, MarcosJiménez Aguilar, PatriciaPasquau, Juanhttp://hdl.handle.net/10498/405172026-10-31T05:03:04Z2026-01-01T00:00:00ZA matched case-control study on Escherichia coli factors contributing to sepsis and septic shock in bacteraemic patients
Maldonado, Natalia; López-Hernández, Inmacualda; Valik, John Karlsson; López Cortés, Luis Eduardo; Martínez Pérez-Crespo, Pedro María; Garcia-Montaner, Andrea; Alcalde-Rico, Manuel; Sousa Dominguez, Adrián; Jover Sáenz, Alfredo; Goikoetxea Aguirre, Ane Josune; Pulido Navazo, Ángeles; Buzon-Martin, Luis; Aller, Ana Isabel; Boix Palop, Lucía; del Arco Jiménez, Alfonso; Smithson Amat, Alejandro; Sánchez Calvo, Juan Manuel; Natera Kindelán, Clara; Reguera Iglesias, José María; Armiñanzas Castillo, Carlos; Galán Sánchez, Fátima; Bahamonde Carrasco, Alberto; Gea Lázaro, Isabel; Castello-Abietar, Cristian; Pérez Camacho, Inés; Marrodán Ciordia, Teresa; Becerril-Carral, Berta; Naucler, Pontus; Pascual-Hernandez, Álvaro; Rodríguez Baño, Jesús; López Hernández, Inmaculada; Retamar Gentil, Pilar; Pascual, Álvaro; Lanz García, Joaquín; León Jiménez, Eva; Corzo-Delgado, Juan Enrique; Pérez Rodríguez, María Teresa; Barcenilla Gaite, Fernando; Montejo Baranda, Miguel; Cuquet Pedragosa, Jordi; Labayru Echeverria, Cristina; Mantecon Vallejo, María Ángeles; Boix Palop, Lucía; Calbo Sebastian, Esther; Farinas Alvarez, María Del Carmen; Fernández Suárez, Jonathan; Arias Temprano, Marta; Canueto Quintero, Jesús; Reyes Bertos, Armando; Sánchez Porto, Antonio; Vinuesa García, David; Guzmán García, Marcos; Jiménez Aguilar, Patricia; Pasquau, Juan
BackgroundOne third of patients with Escherichia coli bacteraemia develop a dysregulated inflammatory response (sepsis/septic shock). Our objective was to investigate whether specific microbiological determinants of E. coli are associated to presentation with sepsis/shock.MethodsA matched case-control study was performed; 101 case-patients with E. coli bacteraemia presenting with sepsis (SEPSIS-3 criteria) and 101 control-patients with E. coli bacteraemia without sepsis were matched by service, sex, age, Charlson index, acquisition and source of the bacteraemia and empirical treatment. Whole genome sequencing of E. coli isolates was performed (Illumina MiSeq Inc.). Sequence type, serotype, fimH type, virulence factors, antibiotic resistance genes, plasmid replicons pathogenicity islands and prophages were determined. A multivariate model was built for presentation with sepsis/septic shock using conditional logistic regression. The predictive capacity on the observed data was measured with the area under the ROC curve (AUROC) with 95% confidence intervals (CI).ResultsHere we show that in the multivariate model (adjusted OR; 95% CI), the ST69 clone (7.53; 1.06-35.05) and pic gene (4.38; 1.53-12.54) are associated to presentation with sepsis/shock, while the genes papC (0.30; 0.12-0.74) and fdeC (0.18; 0.03-1.32) show a protective effect. The AUROC of this model is 0.81 (95% CI 0.74-0.87).ConclusionsWe identify E. coli bacterial factors associated with severe clinical presentation in patients with bacteraemia. Further studies would be needed to consider these factors as potential preventive or therapeutic targets.
2026-01-01T00:00:00ZMolecular characterization of consecutive isolates of OXA-48-producing Klebsiella pneumoniae: changes in the virulome using next-generation sequencing (NGS)Rodríguez Pallares, SaludMateo Vargas, M. AlejandraRodríguez Iglesias, Manuel AntonioGalán Sánchez, Fátimahttp://hdl.handle.net/10498/402272026-09-17T04:04:58Z2023-01-01T00:00:00ZMolecular characterization of consecutive isolates of OXA-48-producing Klebsiella pneumoniae: changes in the virulome using next-generation sequencing (NGS)
Rodríguez Pallares, Salud; Mateo Vargas, M. Alejandra; Rodríguez Iglesias, Manuel Antonio; Galán Sánchez, Fátima
Little is known about the clonality of consecutive OXA-48 producing-Klebsiella pneumoniae isolates from
the same patient and the possibility of changes in their virulomes over time. We studied the molecular
characteristics of twenty OXA-48-producing K. pneumoniae consecutive isolates from six patients using
whole-genome sequencing. The genomes were screened for antimicrobial resistance and virulence factor genes and for replicon groups. MLST and SNPs analysis was performed.
MLST analysis found 3 STs: ST11 (n ¼ 13; 65.0%); ST4975 (n ¼ 5, 25.0%); ST307 (n ¼ 2; 10.0%). AcrAb efflux pump, siderophore enterobactin and rcsAB capsule synthesis regulator were detected in all sequenced isolates. The regulator of mucoid phenotype A (rmpA) and rmpA2 were not detected. Isolates
also carried type 3 fimbriae (n ¼ 19; 95.0%), yersiniabactin (n ¼ 15; 75.0%) and type 1 fimbriae (7; 35.0%).
Type 3 fimbriae and yersiniabactin were lost and recovered in consecutive isolates of two patients, probably acquired by horizontal gene transfer. Our findings reveal that recurrent infections are due to the same isolate, with an average of 2.69 SNPs per month, with different virulence profiles, and that the acquisition of virulence factor genes over time is possible.
2023-01-01T00:00:00ZA prospective survey of Aspergillus spp. in respiratory tract samples: Species identification and susceptibility patternsCastro, CarmenGalán Sánchez, FátimaLinares, Mª JoséTejero, RocíoRuiz, MaiteSerrano, Mª LuisaRodríguez Iglesias, Manuel AntonioMartín-Mazuelos, Estrellahttp://hdl.handle.net/10498/402242026-09-15T04:04:55Z2019-01-01T00:00:00ZA prospective survey of Aspergillus spp. in respiratory tract samples: Species identification and susceptibility patterns
Castro, Carmen; Galán Sánchez, Fátima; Linares, Mª José; Tejero, Rocío; Ruiz, Maite; Serrano, Mª Luisa; Rodríguez Iglesias, Manuel Antonio; Martín-Mazuelos, Estrella
We analyzed the species distribution and susceptibility patterns of 433 strains of Aspergillus spp. isolated from respiratory samples of 419 in-patients included in multicenter prospective study (FUNGAE-IFI) between July 2014 and October 2015. Identification was carried out by conventional methods at each participating center and by molecular sequencing of a portion of the β-tubulin gene at one of the centers. In vitro susceptibility was evaluated by broth microdilution methods and using the E-test (for cryptic species). Species identified included 249 A. fumigatus sensu stricto, 60 A. terreus sensu stricto, 47 A. flavus sensu stricto, 44 A. tubingensis, 18 A. niger sensu stricto , five A. nidulans sensu stricto, three A. tamarii, two A. calidoustus, two A. carneus, one A. acuelatus, one A. carbonarius, and one A. sydowii. Cryptic species were found in 12.5% of isolates (n = 54). The frequency of non-wild-type isolates for amphotericin B was 3.4% (n = 15) of the isolates tested and for azoles 3% (n = 10). None of the Aspergillus spp. were non-wild type to echinocandins. Of the 54 cryptic species only two strains were non-wild-type strains by microdilution method (3.7%) (two A. tubingensis, one to amphotericin B and another one to voriconazole) and by E-test method five strains of A. tubingensis showed high minimal inhibitory concentration (MIC) to amphotericin B (11.4%) and five to azoles (12.1%), one A. calidoustus strain showed high MICs for three azoles (50%), A. carneus to itraconazole (100%) and A. sydowii to amphotericin B and itraconazole (100%). These results provide relevant information on susceptibility patterns, frequency, and epidemiology of species involved in respiratory tract samples and of the incidence of recently described cryptic species.
2019-01-01T00:00:00Z